Abstract
Spitznagel, John K. (University of North Carolina, Chapel Hill). Normal serum cytotoxicity for P32-labeled smooth Enterobacteriaceae. II. Fate of macromolecular and lipid phosphorus of damaged cells. J. Bacteriol. 91:148–152. 1966.—Fresh, normal adult guinea pig serum released micro- and macromolecular forms of metabolically incorporated P32 from smooth Escherichia coli. A significant amount of P32-labeled phospholipid was also released. The overall acid solubility distribution of the P32 label in bacterial suspensions was very little affected in fresh serum, although P32-labeled cytoplasmic contents and cell walls of E. coli lost some P32 when they were suspended in fresh or heat-inactivated normal serum. These findings confirmed and extended earlier evidence that the peripheral wall and membrane structures of smooth E. coli are extensively damaged by factors in fresh normal serum.
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Selected References
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