Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1966 Jan;91(1):205–212. doi: 10.1128/jb.91.1.205-212.1966

Adenovirus Antibody Measured by the Passive Hemagglutination Test

Stanley S Lefkowitz a,1, Julia A Williams a, Bernard E Howard a, M Michael Sigel a
PMCID: PMC315933  PMID: 5903093

Abstract

Lefkowitz, Stanley S. (Variety Children's Research Foundation, Miami, Fla.), Julia A. Williams, Bernard E. Howard, and M. Michael Sigel. Adenovirus antibody measured by the passive hemagglutination test. J. Bacteriol. 91:205–212. 1966.—Rabbits immunized intravenously with adenovirus type 5 antigen were tested for antibody titers by use of the passive hemagglutination test (PHA). Primary and secondary responses were studied, and the class of antibody was determined by means of density gradient centrifugation and reduction with 2-mercaptoethanol (ME). It was found that the PHA was 10 to 100 times more sensitive than complement-fixation and neutralization tests for the detection of antibodies to adenovirus. The immunological response to primary immunization was dependent on the dose of antigen, with antibody appearing in as early as 3 days. After secondary stimulation with the same antigen, there was a rapid response which appeared to be less dose-dependent. It was found that a heavy 19S antibody sensitive to ME was produced early and was followed by a lighter, presumably 7S, ME-resistant antibody. Upon secondary stimulation, both 7S and 19S antibody increased to levels greater than those of the primary injection.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENEDICT A. A., O'BRIEN E. A passive hemagglutination reaction for psittacosis. J Immunol. 1958 Feb;80(2):94–99. [PubMed] [Google Scholar]
  2. DEUTSCH H. F., MORTON J. I. Dissociation of human serum macroglobulins. Science. 1957 Mar 29;125(3248):600–601. doi: 10.1126/science.125.3248.600. [DOI] [PubMed] [Google Scholar]
  3. FRIEDMAN M., BENNETT C. R. A hemagglutination test for detection of adenovirus antibodies. Proc Soc Exp Biol Med. 1957 Apr;94(4):712–717. doi: 10.3181/00379727-94-23062. [DOI] [PubMed] [Google Scholar]
  4. McKENNA J. M., ZUSCHEK F., FRANKEL J. W. An hemagglutination test for titration of antibodies to polioviruses. Proc Soc Exp Biol Med. 1958 Jan;97(1):160–163. doi: 10.3181/00379727-97-23675. [DOI] [PubMed] [Google Scholar]
  5. PEREIRA H. G., KELLY B. Latent infection of rabbits by adenovirus type 5. Nature. 1957 Sep 21;180(4586):615–616. doi: 10.1038/180615b0. [DOI] [PubMed] [Google Scholar]
  6. PRIER J. E., LEBEAU R. W. Studies on the serologic identification of adenovirus. I. Comparison of the hemagglutination, conglutinating complement adsorption, and complement fixation tests. J Lab Clin Med. 1958 Mar;51(3):495–500. [PubMed] [Google Scholar]
  7. ROSS E., GINSBERG H. S. Hemagglutination with adenoviruses; nature of viral antigen. Proc Soc Exp Biol Med. 1958 Jul;98(3):501–505. doi: 10.3181/00379727-98-24089. [DOI] [PubMed] [Google Scholar]
  8. SCOTT L. V., FELTON F. G., BARNEY J. A. Hemagglutination with herpes simplex virus. J Immunol. 1957 Mar;78(3):211–213. [PubMed] [Google Scholar]
  9. STAVITSKY A. B. Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells. J Immunol. 1954 May;72(5):360–367. [PubMed] [Google Scholar]
  10. SVEHAG S. E., MANDEL B. THE FORMATION AND PROPERTIES OF POLIOVIRUS-NEUTRALIZING ANTIBODY. I. 19S AND 7S ANTIBODY FORMATION: DIFFERENCES IN KINETICS AND ANTIGEN DOSE REQUIREMENT FOR INDUCTION. J Exp Med. 1964 Jan 1;119:1–19. doi: 10.1084/jem.119.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SVEHAG S. E., MANDEL B. THE FORMATION AND PROPERTIES OF POLIOVIRUS-NEUTRALIZING ANTIBODY. II. 19S AND 7S ANTIBODY FORMATION: DIFFERENCES IN ANTIGEN DOSE REQUIREMENT FOR SUSTAINED SYNTHESIS, ANAMNESIS, AND SENSITIVITY TO X-IRRADIATION. J Exp Med. 1964 Jan 1;119:21–39. doi: 10.1084/jem.119.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. UHR J. W., FINKELSTEIN M. S. Antibody formation. IV. Formation of rapidly and slowly sedimenting antibodies and immunological memory to bacteriophage phi-X 174. J Exp Med. 1963 Mar 1;117:457–477. doi: 10.1084/jem.117.3.457. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES