Abstract
Oh, J. O. (University of British Columbia, Vancouver, B.C., Canada), and E. J. Gill. Role of interferon-like viral inhibitor in endotoxin-induced corneal resistance to Newcastle disease virus. J. Bacteriol. 91:251–256. 1966.—A state of marked resistance to the toxic corneal effects of Newcastle disease virus (NDV) was observed in rabbit eyes after intravenous injection of 10 or 100 μg of typhoid endotoxin. By use of tissue cultures of rabbit corneal endothelial cells for assay, high titers of an interferon-like viral inhibitor were detected in serum and in ocular aqueous humor of these animals. The pretreatment of eyes with aqueous humor or serum containing the inhibitor markedly suppressed the production of corneal toxicity by NDV. The intravenous injection of 1 μg of the endotoxin had a negligible effect on the corneal reaction, and little or no inhibitor was found in either serum or aqueous humor. Normal aqueous humor or serum contained no inhibitor and had no suppressive effect on the NDV-induced reactions. The results indicated that the inhibitor played an important role in the induction of corneal resistance to NDV in vivo. The inhibitor in aqueous humor of endotoxin-injected rabbits was found to be derived from blood after an increase in the permeability of “blood-aqueous barrier” of iris due to the endotoxin. Therefore, intravenously administered typhoid endotoxin induced corneal resistance to NDV in rabbits through its dual action on host: (i) release of an interferon-like viral inhibitor into the blood stream and (ii) disruption of the blood-aqueous barrier of the iris, thus allowing the passage of the viral inhibitor from blood into the anterior chamber, where it modified the corneal endothelial cells to render them resistant to NDV.
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Selected References
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- BOLIN V. S., ANDERSON J. A., LEYMASTER G. R. Experimental intraocular infection of guinea pigs with mumps virus. Proc Soc Exp Biol Med. 1950 Oct;75(1):166–170. doi: 10.3181/00379727-75-18133. [DOI] [PubMed] [Google Scholar]
- DOUGHERTY R. M., GROUPE V. Studies on nonspecific acquired resistance to viral toxicity in mice. Proc Soc Exp Biol Med. 1957 Jul;95(3):593–596. doi: 10.3181/00379727-95-23303. [DOI] [PubMed] [Google Scholar]
- ECKMAN P. L., KING W. M., BRUNSON J. G. Studies on the blood brain barrier. I. Effects produced by a single injection of gramnegative endotoxin on the permeability of the cerebral vessels. Am J Pathol. 1958 Jul-Aug;34(4):631–643. [PMC free article] [PubMed] [Google Scholar]
- FINKELSTEIN R. A. Alteration of susceptibility of embryonated eggs to Newcastle disease virus by Escherichia coli and endotoxin. Proc Soc Exp Biol Med. 1961 Mar;106:481–484. doi: 10.3181/00379727-106-26376. [DOI] [PubMed] [Google Scholar]
- GILBERT R. P. Mechanisms of the hemodynamic effects of endotoxin. Physiol Rev. 1960 Apr;40:245–279. doi: 10.1152/physrev.1960.40.2.245. [DOI] [PubMed] [Google Scholar]
- GINSBERG H. S., WEDGWOOD R. J. Properdin and the inactivation of viruses. Ann N Y Acad Sci. 1960 Jan 13;83:528–532. doi: 10.1111/j.1749-6632.1960.tb40926.x. [DOI] [PubMed] [Google Scholar]
- GLEDHILL A. W. Sparing effect of serum from mice treated with endotoxin upon certain murine virus diseases. Nature. 1959 Jan 17;183(4655):185–186. doi: 10.1038/183185a0. [DOI] [PubMed] [Google Scholar]
- HO M. INTERFERON-LIKE VIRAL INHIBITOR IN RABBITS AFTER INTRAVENOUS ADMINISTRATION OF ENDOTOXIN. Science. 1964 Dec 11;146(3650):1472–1474. doi: 10.1126/science.146.3650.1472. [DOI] [PubMed] [Google Scholar]
- ISAACS A. INTERFERON. Adv Virus Res. 1963;10:1–38. [PubMed] [Google Scholar]
- KARZON D. T., BUSSELL R. H. Studies of the nonspecific inactivation of Newcastle disease virus by mammalian sera. Ann N Y Acad Sci. 1960 Jan 13;83:539–550. doi: 10.1111/j.1749-6632.1960.tb40928.x. [DOI] [PubMed] [Google Scholar]
- NEMES M. M., HILLEMAN M. R. Effect of Westphal lipid A on viral activities in mice and hamsters. Proc Soc Exp Biol Med. 1962 Jul;110:500–504. doi: 10.3181/00379727-110-27563. [DOI] [PubMed] [Google Scholar]
- OH J. O., EVANS C. A. Suppressive effects of pyrilamine maleate and d-lysergic acid diethylamide (LSD-25) on early corneal lesions produced in vitro by Newcastle disease virus (NDV) and compound 48/80. Virology. 1960 Jan;10:127–143. doi: 10.1016/0042-6822(60)90011-8. [DOI] [PubMed] [Google Scholar]
- OH J. O. Newcastle disease virus (NDV) in tissue cultures of rabbit corneal endothelium: viral multiplication and cytopathogenicity. Br J Exp Pathol. 1961 Oct;42:424–432. [PMC free article] [PubMed] [Google Scholar]
- OH J. O. THE EFFECT OF BACTERIAL ENDOTOXINS ON TOXIC CORNEAL LESIONS PRODUCED BY INFLUENZA AND NEWCASTLE DISEASE VIRUSES. Am J Pathol. 1965 Feb;46:177–195. [PMC free article] [PubMed] [Google Scholar]
- STINEBRING W. R., YOUNGNER J. S. PATTERNS OF INTERFERON APPEARANCE IN MICE INFECTED WITH BACTERIA OR BACTERIAL ENDOTOXIN. Nature. 1964 Nov 14;204:712–712. doi: 10.1038/204712a0. [DOI] [PubMed] [Google Scholar]
- WAGNER R. R., SNYDER R. M., HOOK E. W., LUTTRELL C. N. Effect of bacterial endotoxin on resistance of mice to viral encephalitides; including comparative studies of the interference phenomenon. J Immunol. 1959 Jul;83(1):87–98. [PubMed] [Google Scholar]
- WILCOX W. C., WOOD E. M., OH J. O., EVERETT N. B., EVANS C. A. Morphological and functional changes in corneal endothelium caused by the toxic effects of influenza and Newcastle disease viruses. Br J Exp Pathol. 1958 Dec;39(6):601–609. [PMC free article] [PubMed] [Google Scholar]