Abstract
Schlosser, Eckart (University of Illinois, Urbana), and David Gottlieb. Sterols and the sensitivity of Pythium species to filipin. J. Bacteriol. 91:1080–1084. 1966.—The growth of several Pythium species was not affected by filipin. No leakage of inorganic phosphate was observed after treatment with the antibiotic. No sterol could be detected in 1 g (dry weight) of mycelium. Thus, the insensitivity of these fungi to the antibiotic may be explained by the lack of sterols, the postulated reaction site for filipin in the cell membrane. Though not capable of synthesizing sterols, Pythium species can incorporate exogeneous sterols, which renders them sensitive to filipin; such treatment causes a lag in growth and leakage of inorganic phosphate. The leakage after filipin treatment is indirect evidence that the sterols have been incorporated into the cell membrane. Induced sensitivity to filipin was reversible; it was lost when the sterols were diluted out by one transfer through a medium free from sterols. The hypothesis that the primary site of interaction of filipin is the sterol located in the cell membrane was strengthened by these studies. The experiments further demonstrated a change in sensitivity of a fungus to a toxic agent due to nutritional conditions.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- APPLETON G. S., KIEBER R. J., PAYNE W. J. The sterol content of fungi. II. Screening of representative yeasts and molds for sterol content. Appl Microbiol. 1955 Jul;3(4):249–251. doi: 10.1128/am.3.4.249-251.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FEINGOLD D. S. THE ACTION OF AMPHOTERICIN B ON MYCOPLASMA LAIDLAWII. Biochem Biophys Res Commun. 1965 Apr 9;19:261–267. doi: 10.1016/0006-291x(65)90515-2. [DOI] [PubMed] [Google Scholar]
- GOTTLIEB D., CARTER H. E., SLONEKER J. H., AMMANN A. Protection of fungi against polyene antibiotics by sterols. Science. 1958 Aug 15;128(3320):361–361. doi: 10.1126/science.128.3320.361. [DOI] [PubMed] [Google Scholar]
- HENDRIX J. W. STEROL INDUCTION OF REPRODUCTION AND STIMULATION OF GROWTH OF PYTHIUM AND PHYTOPHTHORA. Science. 1964 May 22;144(3621):1028–1029. doi: 10.1126/science.144.3621.1028. [DOI] [PubMed] [Google Scholar]
- WEBER M. M., KINSKY S. C. EFFECT OF CHOLESTEROL ON THE SENSITIVITY OF MYCOPLASMA LAIDLAWII TO THE POLYENE ANTIBIOTIC FILIPIN. J Bacteriol. 1965 Feb;89:306–312. doi: 10.1128/jb.89.2.306-312.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]