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. 1966 Mar;91(3):1098–1104. doi: 10.1128/jb.91.3.1098-1104.1966

Identification of Nutritional Components in Trypticase Responsible for Recovery of Escherichia coli Injured by Freezing1

C Wayne Moss a,2, M L Speck a
PMCID: PMC316001  PMID: 5326093

Abstract

Moss, C. Wayne (North Carolina State University, Raleigh), and M. L. Speck. Identification of nutritional components in Trypticase responsible for recovery of Escherichia coli injured by freezing. J. Bacteriol. 91:1098–1104. 1966.—Freezing and storage of Escherichia coli at −20 C resulted in nonlethal or “metabolic” injury to a proportion of the surviving population. The injury was manifested as an increased nutritional requirement after freezing. Injured cells could not grow on a minimal agar medium, but could develop on Trypticase Soy Agar. The percentage of injured survivors varied among strains, but was little affected by altering the freezing menstruum. Trypticase was found to be the component in Trypticase Soy Agar responsible for the recovery of injured cells, and contained five closely related peptides that possessed most of the biological activity. Isolation of the peptides was accomplished by Sephadex gel chromatography, paper chromatography, and high-voltage paper electrophoresis. Hydrolysis of the peptides destroyed the ability to restore injured cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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