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. 1966 Mar;91(3):1218–1223. doi: 10.1128/jb.91.3.1218-1223.1966

Effects of α-Glycerophosphate and of Palmityl-Coenzyme A on Lipid Synthesis in Yeast Extracts

David White a,1, Harold P Klein a
PMCID: PMC316016  PMID: 5929752

Abstract

White, David (Ames Research Center, Moffett Field, Calif.), and Harold P. Klein. Effects of α-glycerophosphate and of palmityl-coenzyme A on lipid synthesis in yeast extracts. J. Bacteriol. 91:1218–1223. 1966.—The incorporation of acetate into fatty acids, but not into nonsaponifiable lipids, was stimulated by α-glycerophosphate in a supernatant fraction of Saccharomyces cerevisiae, obtained after centrifugation at 86,000 × g for 60 min. There was a pronounced effect at concentrations below 2 mm, but at concentrations above 5 mm α-glycerophosphate was relatively less stimulatory. α-Glycerophosphate markedly increased the percentage of esterified fatty acids among the products, and the formation of both saturated and unsaturated fatty acids was stimulated. Palmityl-coenzyme A inhibited fatty acid synthesis, affecting the formation of unsaturated acids more severely than saturated acids. In the presence of sufficient α-glycerophosphate to alleviate these inhibitions, palmityl-coenzyme A still reduced the formation of certain unsaturated fatty acids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABRAHAM S., CHAIKOFF I. L. Glycolytic pathways and lipogenesis in mammary glands of lactating and nonlactating normal rats. J Biol Chem. 1959 Sep;234:2246–2253. [PubMed] [Google Scholar]
  2. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  3. BORTZ W. M., LYNEN F. THE INHIBITION OF ACETYL COA CARBOXYLASE BY LONG CHAIN ACYL COA DERIVATIVES. Biochem Z. 1963 Aug 14;337:505–509. [PubMed] [Google Scholar]
  4. GIBSON D. M., JACOB M. I., PORTER J. W., TIETZ A., WAKIL S. J. Biosynthesis of fatty acids by soluble enzyme fractions. Biochim Biophys Acta. 1957 Jan;23(1):219–220. doi: 10.1016/0006-3002(57)90319-0. [DOI] [PubMed] [Google Scholar]
  5. HIRSCH P. F., BARUCH H., CHAIKOFF I. L. The relation of glucose oxidation to lipogenesis in mammary tissue. J Biol Chem. 1954 Oct;210(2):785–797. [PubMed] [Google Scholar]
  6. HOWARD C. F., LOWENSTEIN J. M. THE EFFECT OF ALPH-GLYCEROPHOSPHATE ON THE MICROSOMAL STIMULATION OF FATTY ACID SYNTHESIS. Biochim Biophys Acta. 1964 Apr 20;84:226–228. doi: 10.1016/0926-6542(64)90089-7. [DOI] [PubMed] [Google Scholar]
  7. KLEIN H. P. STIMULATION OF LIPID SYNTHESIS BY YEAST RIBOSOMAL PREPARATIONS. Biochim Biophys Acta. 1963 Oct 22;70:606–608. doi: 10.1016/0006-3002(63)90803-5. [DOI] [PubMed] [Google Scholar]
  8. KLEIN H. P. Some observations on a cell free lipid synthesizing system from Saccharomyces cerevisiae. J Bacteriol. 1957 Apr;73(4):530–543. doi: 10.1128/jb.73.4.530-537.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KORNBERG A., PRICER W. E., Jr Enzymatic synthesis of the coenzyme A derivatives of long chain fatty acids. J Biol Chem. 1953 Sep;204(1):329–343. [PubMed] [Google Scholar]
  10. PERRY W. F. Acetate utilization by liver and adipose tissue of rats fasted in the cold. Can J Biochem Physiol. 1958 Feb;36(2):237–241. [PubMed] [Google Scholar]
  11. PORTER J. W., LONG R. W. A study of the role of palmityl coenzyme A in fatty acid synthesis by the pigeon liver system. J Biol Chem. 1958 Jul;233(1):20–25. [PubMed] [Google Scholar]
  12. SHAW W. N., DITURI F., GURIN S. Lipogenesis in particle-free extracts of rat liver. II. Experimental diabetes. J Biol Chem. 1957 May;226(1):417–425. [PubMed] [Google Scholar]
  13. STUMPF P. K. Lipid metabolism. Annu Rev Biochem. 1960;29:261–294. doi: 10.1146/annurev.bi.29.070160.001401. [DOI] [PubMed] [Google Scholar]
  14. VAGELOS P. R., ALBERTS A. W., MARTIN D. B. Studies on the mechnism of activation of acetyl coenzyme A carboxylase by citrate. J Biol Chem. 1963 Feb;238:533–540. [PubMed] [Google Scholar]
  15. VAGELOS P. R. LIPID METABOLISM. Annu Rev Biochem. 1964;33:139–172. doi: 10.1146/annurev.bi.33.070164.001035. [DOI] [PubMed] [Google Scholar]
  16. WHITE D., KLEIN H. P. FACTORS AFFECTING FATTY ACID SYNTHESIS IN CELL-FREE PREPARATIONS FROM SACCHAROMYCES CEREVISIAE. Biochem Biophys Res Commun. 1965 Jun 18;20:78–84. doi: 10.1016/0006-291x(65)90953-8. [DOI] [PubMed] [Google Scholar]

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