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. 1966 Mar;91(3):1248–1256. doi: 10.1128/jb.91.3.1248-1256.1966

Immunofluorescence in Cells Derived from Burkitt's Lymphoma

Gertrude Henle a, Werner Henle a,1
PMCID: PMC316020  PMID: 4160230

Abstract

Henle, Gertrude (Children's Hospital of Philadelphia, Philadelphia, Pa.), and Werner Henle. Immunofluorescence in cells derived from Burkitt's lymphoma. J. Bacteriol. 91:1248–1256. 1966.—Indirect immunofluorescence tests led to the brilliant staining of a small proportion of the cells in five different cultures derived from Burkitt's (African) lymphomas. The reaction was not restricted to the 17 sera from cases of this disease but extended to many sera from American individuals, whether healthy donors or patients suffering from a variety of illnesses. The incidence of positive sera increased with age from about 30% in childhood to > 90% in adults. Fluorescein-isothiocyanate-conjugated human γ-globulins were suitable for direct staining of the same proportion of cells. The stained cells appeared to be in varying stages of degeneration, but cultural conditions leading to an increase in the cellular death rates failed to result in a rise in fluorescent cells. Several observations suggest that the stainable cells might be those which are seen to harbor virus particles under the electron microscope. Two cell lines derived from leukemic patients in this country also contained a small fraction of stainable cells but two others, and numerous primary human leukocyte cultures, gave consistently negative results. Attempts to relate the staining to known viral antigens have failed to implicate herpes simplex, varicella, cytomegalo, and reo viruses types 1, 2, and 3. The nature of the virus carried by the lymphoma cells as well as of the staining reactions remains to be determined.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong D., Henle G., Henle W. Complement-fixation tests with cell lines derived from Burkitt's lymphoma and acute leukemias. J Bacteriol. 1966 Mar;91(3):1257–1262. doi: 10.1128/jb.91.3.1257-1262.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BELL T. M., MASSIE A., ROSS M. G., WILLIAMS M. C. ISOLATION OF A REOVIRUS FROM A CASE OF BURKITT'S LYMPHOMA. Br Med J. 1964 May 9;1(5392):1212–1213. doi: 10.1136/bmj.1.5392.1212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COONS A. H., KAPLAN M. H. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med. 1950 Jan 1;91(1):1–13. doi: 10.1084/jem.91.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. EPSTEIN M. A., ACHONG B. G., BARR Y. M. VIRUS PARTICLES IN CULTURED LYMPHOBLASTS FROM BURKITT'S LYMPHOMA. Lancet. 1964 Mar 28;1(7335):702–703. doi: 10.1016/s0140-6736(64)91524-7. [DOI] [PubMed] [Google Scholar]
  5. EPSTEIN M. A., BARR Y. M., ACHONG B. G. A SECOND VIRUS-CARRYING TISSUE CULTURE STRAIN (EB2) OF LYMPHOBLASTS FROM BURKITT'S LYMPHOMA. Pathol Biol. 1964 Dec;12:1233–1234. [PubMed] [Google Scholar]
  6. EPSTEIN M. A., BARR Y. M., ACHONG B. G. THE BEHAVIOUR AND MORPHOLOGY OF A SECOND TISSUE CULTURE STRAIN (EB2) OF LYMPHOBLASTS FROM BURKITT'S LYMPHOMA. Br J Cancer. 1965 Mar;19:108–115. doi: 10.1038/bjc.1965.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. EPSTEIN M. A., BARR Y. M. CHARACTERISTICS AND MODE OF GROWTH OF TISSUE CULTURE STRAIN (EB1) OF HUMAN LYMPHOBLASTS FROM BURKITT'S LYMPHOMA. J Natl Cancer Inst. 1965 Feb;34:231–240. doi: 10.1093/jnci/34.2.231. [DOI] [PubMed] [Google Scholar]
  8. EPSTEIN M. A., BARR Y. M. CULTIVATION IN VITRO OF HUMAN LYMPHOBLASTS FROM BURKITT'S MALIGNANT LYMPHOMA. Lancet. 1964 Feb 1;1(7327):252–253. doi: 10.1016/s0140-6736(64)92354-2. [DOI] [PubMed] [Google Scholar]
  9. EPSTEIN M. A., HENLE G., ACHONG B. G., BARR Y. M. MORPHOLOGICAL AND BIOLOGICAL STUDIES ON A VIRUS IN CULTURED LYMPHOBLASTS FROM BURKITT'S LYMPHOMA. J Exp Med. 1965 May 1;121:761–770. doi: 10.1084/jem.121.5.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FINK M. A., MALMGREN R. A., RAUSCHER F. J., ORR H. C., KARON M. APPLICATION OF IMMUNOFLUORESCENCE TO THE STUDY OF HUMAN LEUKEMIA. J Natl Cancer Inst. 1964 Sep;33:581–588. [PubMed] [Google Scholar]
  11. HENLE G., HENLE W. CROSS-REACTIONS AMONG GAMMA-GLOBULINS OF VARIOUS SPECIES IN INDIRECT IMMUNOFLUORESCENCE. J Immunol. 1965 Jul;95:118–124. [PubMed] [Google Scholar]
  12. HENLEGHENLE W. EVIDENCE FOR A PERSISTENT VIRAL INFECTION IN A CELL LINE DERIVED FROM BURKITT'S LYMPHOMA. J Bacteriol. 1965 Jan;89:252–258. doi: 10.1128/jb.89.1.252-258.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. NEMES M. M., HILLEMAN M. R. EFFECTIVE TREATMENT OF EXPERIMENTAL HERPES SIMPLEX KERATITIS WITH NEW DERIVATIVE, 5-METHYLAMINO-2'-DEOXYURIDINE (MADU). Proc Soc Exp Biol Med. 1965 Jun;119:515–520. doi: 10.3181/00379727-119-30224. [DOI] [PubMed] [Google Scholar]
  14. NOWELL P. C. Inhibition of human leukocyte mitosis by prednisolone in vitro. Cancer Res. 1961 Dec;21:1518–1521. [PubMed] [Google Scholar]
  15. O'CONOR G. T., RAPPAPORT H., SMITH E. B. CHILDHOOD LYMPHOMA RESEMBLING "BURKITT TUMOR" IN THE UNITED STATES. Cancer. 1965 Apr;18:411–417. doi: 10.1002/1097-0142(196504)18:4<411::aid-cncr2820180403>3.0.co;2-5. [DOI] [PubMed] [Google Scholar]
  16. PULVERTAFT J. V. CYTOLOGY OF BURKITT'S TUMOUR (AFRICAN LYMPHOMA). Lancet. 1964 Feb 1;1(7327):238–240. doi: 10.1016/s0140-6736(64)92345-1. [DOI] [PubMed] [Google Scholar]
  17. RIGGS J. L., SEIWALD R. J., BURCKHALTER J. H., DOWNS C. M., METCALF T. G. Isothiocyanate compounds as fluorescent labeling agents for immune serum. Am J Pathol. 1958 Nov-Dec;34(6):1081–1097. [PMC free article] [PubMed] [Google Scholar]
  18. RODRIGUEZ J., DEINHARDT F. Preparation of a semipermanent mounting medium for fluorescent antibody studies. Virology. 1960 Oct;12:316–317. doi: 10.1016/0042-6822(60)90205-1. [DOI] [PubMed] [Google Scholar]
  19. SMOLENS J., STOKES J., Jr, VOGT A. B. Human plasmapheresis and its effect on antibodies. J Immunol. 1957 Nov;79(5):434–439. [PubMed] [Google Scholar]
  20. STEWART S. E., LOVELACE E., WHANG J. J., NGU V. A. BURKITT TUMOR: TISSUE CULTURE, CYTOGENETIC AND VIRUS STUDIES. J Natl Cancer Inst. 1965 Feb;34:319–327. doi: 10.1093/jnci/34.2.319. [DOI] [PubMed] [Google Scholar]
  21. WELLER T. H., COONS A. H. Fluorescent antibody studies with agents of varicella and herpes zoster propagated in vitro. Proc Soc Exp Biol Med. 1954 Aug-Sep;86(4):789–794. doi: 10.3181/00379727-86-21235. [DOI] [PubMed] [Google Scholar]

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