Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1966 Mar;91(3):1327–1332. doi: 10.1128/jb.91.3.1327-1332.1966

Bacteriophage Infecting the Myxobacterium Chondrococcus columnaris

David T Kingsbury a,1, Erling J Ordal a
PMCID: PMC316032  PMID: 5929758

Abstract

Kingsbury, David T. (University of Washington, Seattle), and Erling J. Ordal. Bacteriophage infecting the myxobacterium Chondrococcus columnaris. J. Bacteriol. 91:1327–1332. 1966.—During a series of screening experiments, seven bacteriophages which infect the pathogenic myxobacterium Chondrococcus columnaris were isolated. Of these, one was chosen for detailed study. This phage has a wide host range among strains of C. columnaris, but does not infect other myxobacterial species tested. Morphologically, this phage resembles coliphage T2, though it is smaller. It has a head diameter of 600 A, a tail length of 1,000 A, and a tail width of 200 A. The head is attached to the tail by a well-defined neck. The turbid plaques produced by this phage are similar in appearance to those produced by coliphage λ, and average 1 mm in diameter. The phage has a latent period of 100 min, a rise period of an additional 90 min, and a burst size of 23. Calcium ions at a concentration of 0.004 m are required for adsorption. This requirement cannot be met by substitution of magnesium ions. A purified preparation of 2 × 1012 phage particles was extracted with phenol, and the nucleic acid was identified as deoxyribonucleic acid (DNA). Base ratios of the phage DNA and the DNA of two propagating strains were similar. Streptomycin at a concentration of 70 μg/ml inhibits phage infection at an early stage, probably by inhibiting injection of the phage DNA.

Full text

PDF
1327

Images in this article

1328Image
on p.1328

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANACKER R. L., ORDAL E. J. Studies on the myxobacterium Chondrococcus columnaris. I. Serological typing. J Bacteriol. 1959 Jul;78(1):25–32. doi: 10.1128/jb.78.1.25-32.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ANACKER R. L., ORDAL E. J. Study of a bacteriophage infecting the myxobacterium Chondrococcus columnaris. J Bacteriol. 1955 Dec;70(6):738–741. doi: 10.1128/jb.70.6.738-741.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BERK R. S. NUTRITIONAL STUDIES ON THE "AUTO-PLAQUE" PHENOMENON IN PSEUDOMONAS AERUGINOSA. J Bacteriol. 1963 Oct;86:728–734. doi: 10.1128/jb.86.4.728-734.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BERTANI G. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol. 1951 Sep;62(3):293–300. doi: 10.1128/jb.62.3.293-300.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BROCK T. D. ACTION OF STREPTOMYCIN AND RELATED ANTIBIOTICS. Fed Proc. 1964 Sep-Oct;23:965–975. [PubMed] [Google Scholar]
  6. BROCK T. D., WOOLEY S. O. STREPTOMYCIN AS AN ANTIVIRAL AGENT: MODE OF ACTION. Science. 1963 Sep 13;141(3585):1065–1067. doi: 10.1126/science.141.3585.1065. [DOI] [PubMed] [Google Scholar]
  7. Fastier L. B. A Bacteriophagelike Principle for Pseudomonas pyocyanea (Pseudomonas aeruginosa). J Bacteriol. 1945 Jun;49(6):633–633. doi: 10.1128/jb.49.6.633-633.1945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GIERER A., SCHRAMM G. Infectivity of ribonucleic acid from tobacco mosaic virus. Nature. 1956 Apr 14;177(4511):702–703. doi: 10.1038/177702a0. [DOI] [PubMed] [Google Scholar]
  9. HERSHEY A. D., CHASE M. Independent functions of viral protein and nucleic acid in growth of bacteriophage. J Gen Physiol. 1952 May;36(1):39–56. doi: 10.1085/jgp.36.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KIRBY K. S. Some new solvent systems for the paper chromatography of nucleic acid degradation products. Biochim Biophys Acta. 1955 Dec;18(4):575–576. doi: 10.1016/0006-3002(55)90157-8. [DOI] [PubMed] [Google Scholar]
  11. LOEB T., ZINDER N. D. A bacteriophage containing RNA. Proc Natl Acad Sci U S A. 1961 Mar 15;47:282–289. doi: 10.1073/pnas.47.3.282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
  13. MASON D. J., POWELSON D. Lysis of Myxococcus xanthus. J Gen Microbiol. 1958 Aug;19(1):65–70. doi: 10.1099/00221287-19-1-65. [DOI] [PubMed] [Google Scholar]
  14. McCLOY E. W. Lysogenicity and immunity to Bacillus phage W. J Gen Microbiol. 1958 Feb;18(1):198–220. doi: 10.1099/00221287-18-1-198. [DOI] [PubMed] [Google Scholar]
  15. NORTHROP J. H., CAVALLERO F. Mutagens and infectious nucleic acids. Proc Natl Acad Sci U S A. 1961 Oct 15;47:1602–1606. doi: 10.1073/pnas.47.10.1602. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES