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. 1966 Mar;91(3):1356–1362. doi: 10.1128/jb.91.3.1356-1362.1966

Formation of a Functional Electron Transport System During Growth of Penicillin-Induced Spheroplasts of Haemophilus parainfluenzae

Elizabeth A Wright 1, David C White 1
PMCID: PMC316035  PMID: 4286451

Abstract

Wright, Elizabeth A. (University of Kentucky College of Medicine, Lexington), and David C. White. Formation of a functional electron transport system during growth of penicillin-induced spheroplasts of Haemophilus parainfluenzae. J. Bacteriol. 91:1356–1362. 1966.—Penicillin in a lactose medium can be used to cause the formation of spheroplasts in Haemophilus parainfluenzae. The resulting spheroplasts grew under conditions which produced rapid formation of the electron transport system in the normal bacteria. The following elements that are incorporated into a functionally active electron transport system were formed in spheroplasts: formate and l-lactate dehydrogenases, 2-demethyl vitamin K2, cytochromes b1 and c1, and the cytochrome oxidases. The catabolic enzymes aldolase, glyceraldehyde-3-phosphate dehydrogenase, and malic dehydrogenase showed slight increases in activity. These experiments indicated that spheroplasts can form a fully functional electron transport system essentially similar to that formed during normal growth. The various components of the electron transport system were formed at different rates in the growing spheroplasts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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