Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1966 Apr;91(4):1403–1409. doi: 10.1128/jb.91.4.1403-1409.1966

Antileptospiral Activity of Serum I. Normal and Immune Serum

Russell C Johnson 1, Louis H Muschel 1
PMCID: PMC316056  PMID: 5929774

Abstract

Johnson, Russell C. (University of Minnesota, Minneapolis), and Louis H. Muschel. Antileptospiral activity of serum. I. Normal and immune serum. J. Bacteriol. 91:1403–1409. 1966.—Normal serum was found to exert a leptospiricidal effect, mediated by the complement system, against the nonpathogenic leptospires. Although resistant to normal serum, the pathogenic serotypes were susceptible to antiserum plus complement. Several variables in these immune leptospiricidal reactions were investigated. A reaction period of 3 hr at 37 C between serum substances and 1-day-old cells provided a maximal leptospiricidal effect. The normal serum of the rabbit, guinea pig, bovine, and human were leptospiricidal against the nonpathogenic serotypes, and, in conjunction with rabbit antiserum, rabbit and bovine complement were leptospiricidal against the pathogenic serotypes. Studies with C14-labeled leptospires indicated that the immune leptospiricidal reaction was associated with a loss of permeability control. Thus, like the gram-negative bacteria, the treponemes, erythrocytes, and nucleated mammalian cells, the leptospires may be included as cell types susceptible to the antibody-complement system.

Full text

PDF
1403

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BABUDIERI B. Laboratory diagnosis of leptospirosis. Bull World Health Organ. 1961;24:45–58. [PMC free article] [PubMed] [Google Scholar]
  2. ELLINGHAUSEN H. C., Jr, MCCULLOUGH W. G. NUTRITION OF LEPTOSPIRA POMONA AND GROWTH OF 13 OTHER SEROTYPES: FRACTIONATION OF OLEIC ALBUMIN COMPLEX AND A MEDIUM OF BOVINE ALBUMIN AND POLYSORBATE 80. Am J Vet Res. 1965 Jan;26:45–51. [PubMed] [Google Scholar]
  3. FUZI M., CSOKA R. Rapid method for the differentiation of parasitic and saprophytic leptospirae. J Bacteriol. 1961 Jun;81:1008–1008. doi: 10.1128/jb.81.6.1008-1008.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FUZI M., CSOKA R. [Differentiation of pathogenic and saprophytic Leptospira with a copper sulfate test]. Zentralbl Bakteriol. 1960 Jun;179:231–237. [PubMed] [Google Scholar]
  5. GREEN H., GOLDBERG B. The action of antibody and complement on mammalian cells. Ann N Y Acad Sci. 1960 May 31;87:352–362. doi: 10.1111/j.1749-6632.1960.tb23205.x. [DOI] [PubMed] [Google Scholar]
  6. JOHNSON R. C., GARY N. D. NUTRITION OF LEPTOSPIRA POMONA. II. FATTY ACID REQUIREMENTS. J Bacteriol. 1963 May;85:976–982. doi: 10.1128/jb.85.5.976-982.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. JOHNSON R. C., MUSCHEL L. H. ANTILEPTOSPIRAL ACTIVITY OF NORMAL SERUM. J Bacteriol. 1965 Jun;89:1625–1626. doi: 10.1128/jb.89.6.1625-1626.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. JOHNSON R. C., ROGERS P. 5-FLUOROURACIL AS A SELECTIVE AGENT FOR GROWTH OF LEPTOSPIRAE. J Bacteriol. 1964 Feb;87:422–426. doi: 10.1128/jb.87.2.422-426.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. JOHNSON R. C., ROGERS P. DIFFERENTIATION OF PATHOGENIC AND SAPROPHYTIC LEPTOSPIRES WITH 8-AZAGUANINE. J Bacteriol. 1964 Dec;88:1618–1623. doi: 10.1128/jb.88.6.1618-1623.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. JOHNSON R. C., WILSON J. B. Nutrition of Leptospira pomona. J Bacteriol. 1960 Sep;80:406–411. doi: 10.1128/jb.80.3.406-411.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LAWRENCE J. J. The lysis of leptospires by antiserum. Aust J Exp Biol Med Sci. 1955 Feb;33(1):91–101. doi: 10.1038/icb.1955.10. [DOI] [PubMed] [Google Scholar]
  12. MUSCHEL L. H. Serum bactericidal actions. Ann N Y Acad Sci. 1960 Nov 21;88:1265–1272. doi: 10.1111/j.1749-6632.1960.tb20117.x. [DOI] [PubMed] [Google Scholar]
  13. YANAGAWA R., HIRAMUNE T., AKAIKE Y. GROWTH OF SAPROPHYTIC AND PATHOGENIC LEPTOSPIRAE ON SOLID MEDIUM IN CARBON DIOXIDE-FREE AIR. J Bacteriol. 1963 Apr;85:953–954. doi: 10.1128/jb.85.4.953-954.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES