Abstract
Anacker, R. L. (Rocky Mountain Laboratory, Hamilton, Mont.), W. D. Bickel, W. T. Haskins, K. C. Milner, E. Ribi, and J. A. Rudbach. Frequency of occurrence of native hapten among enterobacterial species. J. Bacteriol. 91:1427–1433. 1966.—Smooth cultures of representative Enterobacteriaceae were screened for the presence of native hapten, a substance previously extracted with trichloroacetic acid from the protoplasmic fraction of one strain each of Escherichia coli O111:B4 and O113. Trichloroacetic acid extracts of protoplasmic fractions of the cells were analyzed for chemical composition, for constituent sugars by paper chromatography, for immunochemical relationship to endotoxin purified by gel filtration, for sedimentation behavior, and for pyrogenicity in rabbits and lethal toxicity in chick embryos. Extracts from two of three additional strains of E. coli O113, all five additional strains of E. coli O111:B4, and one strain each of E. coli O26:B6 and O55:B5 were similar to previously described native hapten in chemical composition, sedimentation properties (S20,w, 3.7 to 5.2), biological potency (usually less than 0.1% that of corresponding endotoxin), and immunochemical relationship to endotoxin. Extracts of one strain each of E. coli O127:B8, Serratia marcescens, Citrobacter freundii, Salmonella enteritidis, and of two lipopolysaccharide-deficient mutants of S. enteritidis differed from typical native hapten. The biosynthetic relationship of native hapten to endotoxin has not yet been revealed.
Full text
PDF






Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ANACKER R. L., FINKELSTEIN R. A., HASKINS W. T., LANDY M., MILNER K. C., RIBI E., STASHAK P. W. ORIGIN AND PROPERTIES OF NATURALLY OCCURRING HAPTEN FROM ESCHERICHIA COLI. J Bacteriol. 1964 Dec;88:1705–1720. doi: 10.1128/jb.88.6.1705-1720.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ANDREWS P. Estimation of molecular weights of proteins by gel filtration. Nature. 1962 Oct 6;196:36–39. doi: 10.1038/196036a0. [DOI] [PubMed] [Google Scholar]
- Anderson E. H. Growth Requirements of Virus-Resistant Mutants of Escherichia Coli Strain "B". Proc Natl Acad Sci U S A. 1946 May;32(5):120–128. doi: 10.1073/pnas.32.5.120. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BECKMANN I., SUBBAIAH T. V., STOCKER B. A. ROUGH MUTANTS OF SALMONELLA TYPHIMURIUM. II. SEROLOGICAL AND CHEMICAL INVESTIGATIONS. Nature. 1964 Mar 28;201:1299–1301. doi: 10.1038/2011299a0. [DOI] [PubMed] [Google Scholar]
- CYNKIN M. A., ASHWELL G. Estimation of 3-deoxy sugars by means of the malonaldehyde-thiobarbituric acid reaction. Nature. 1960 Apr 9;186:155–156. doi: 10.1038/186155a0. [DOI] [PubMed] [Google Scholar]
- FINKELSTEIN R. A. OBSERVATIONS ON MODE OF ACTION OF ENDOTOXIN IN CHICK EMBRYOS. Proc Soc Exp Biol Med. 1964 Mar;115:702–707. doi: 10.3181/00379727-115-29012. [DOI] [PubMed] [Google Scholar]
- JARVIS F. G., MESENKO M. T., TIBBS K. E. Production of Vi antigen on a chemically defined medium by a coliform bacterium. J Bacteriol. 1960 Nov;80:673–676. doi: 10.1128/jb.80.5.673-676.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KEENE W. R., SILBERMAN H. R., LANDY M. Observations on the pyrogenic response and its application to the bioassay of endotoxin. J Clin Invest. 1961 Feb;40:295–301. doi: 10.1172/JCI104256. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUEDERITZ O., RISSE H. J., SCHULTE-HOLTHAUSEN H., STROMINGER J. L., SUTHERLAND I. W., WESTPHAL O. BIOCHEMICAL STUDIES OF THE SMOOTH-ROUGH MUTATION IN SALMONELLA MINNESOTA. J Bacteriol. 1965 Feb;89:343–354. doi: 10.1128/jb.89.2.343-354.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MILNER K. C., ANACKER R. L., FUKUSHI K., HASKINS W. T., LANDY M., MALMGREN B., RIBI E. SYMPOSIUM ON RELATIONSHIP OF STRUCTURE OF MICROORGANISMS TO THEIR IMMUNOLOGICAL PROPERTIES. III. STRUCTURE AND BIOLOGICAL PROPERTIES OF SURFACE ANTIGENS FROM GRAM-NEGATIVE BACTERIA. Bacteriol Rev. 1963 Dec;27:352–368. doi: 10.1128/br.27.4.352-368.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MILNER K. C., JELLISON W. L., SMITH B. The role of lice in transmission of Salmonella. J Infect Dis. 1957 Sep-Oct;101(2):181–192. doi: 10.1093/infdis/101.2.181. [DOI] [PubMed] [Google Scholar]
- NAKANO M. Mutants of Salmonella with unusually low toxicity for mice. Nature. 1962 Dec 15;196:1118–1119. doi: 10.1038/1961118a0. [DOI] [PubMed] [Google Scholar]
- NIKAIDO H., MIKAIDO K., SUBBAIAH T. V., STOCKER B. A. ROUGH MUTANTS OF SALMONELLA TYPHIMURIUM. III. ENZYMATIC SYNTHESIS OF NUCLEOTIDE-SUGAR COMPOUNDS. Nature. 1964 Mar 28;201:1301–1302. doi: 10.1038/2011301a0. [DOI] [PubMed] [Google Scholar]
- OSBORN M. J. STUDIES ON THE GRAM-NEGATIVE CELL WALL. I. EVIDENCE FOR THE ROLE OF 2-KETO- 3-DEOXYOCTONATE IN THE LIPOPOLYSACCHARIDE OF SALMONELLA TYPHIMURIUM. Proc Natl Acad Sci U S A. 1963 Sep;50:499–506. doi: 10.1073/pnas.50.3.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RIBI E., HASKINS W. T., MILNER K. C., ANACKER R. L., RITTER D. B., GOODE G., TRAPANI R. J., LANDY M. Physicochemical changes in endotoxin associated with loss of biological potency. J Bacteriol. 1962 Oct;84:803–814. doi: 10.1128/jb.84.4.803-814.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SUBBAIAH T. V., STOCKER B. A. ROUGH MUTANTS OF SALMONELLA TYPHIMURIUM. I. GENETICS. Nature. 1964 Mar 28;201:1298–1299. doi: 10.1038/2011298a0. [DOI] [PubMed] [Google Scholar]
- VOLKIN E., COHN W. E. Estimation of nucleic acids. Methods Biochem Anal. 1954;1:287–305. doi: 10.1002/9780470110171.ch11. [DOI] [PubMed] [Google Scholar]
- WARAVDEKAR V. S., SASLAW L. D. A sensitive colorimetric method for the estimation of 2-deoxy sugars with the use of the malonaldehyde-thiobarbituric acid reaction. J Biol Chem. 1959 Aug;234(8):1945–1950. [PubMed] [Google Scholar]