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. 1966 Apr;91(4):1469–1476. doi: 10.1128/jb.91.4.1469-1476.1966

Chromosomal Relocation of Prophage-Associated Bacterial Genes1

M W Taylor a,2, C Yanofsky a
PMCID: PMC316065  PMID: 5326111

Abstract

Taylor, M. W. (Stanford University, Stanford, Calif.), and C. Yanofsky. Chromosomal relocation of prophage-associated bacterial genes. J. Bacteriol. 91:1469–1476. 1966.—Two distinguishable colony types, rough-edged and smooth-edged, were observed when tryptophan auxotrophs of Escherichia coli were transformed to tryptophan independence with DNA from the hybrid nondefective transducing phage iλhφ80T1Stryp A+B+, and with the helper phage λi434. P1kc transduction experiments with cells of the two types of colonies as genetic donors showed that the iλhφ80T1Stryp A+B+ prophage was located at different regions of the E. coli chromosome. In cells of rough-edged colonies, the prophage was linked to the tryp-cys region, its normal location, whereas in cells of smooth-edged colonies the prophage was associated with the gal region. When transformation experiments were performed with a T1Rtryp− deletion mutant as recipient, and phage λi434 as helper, prophage localization was only detected at the gal region. Localization of λhφ80T1Stryp A+B+ prophage near gal does not appear to be due to the formation of a recombinant phage carrying tryp A+B+, but is due to some type of interaction between the genomes of iλhφ80T1Stryp A+B+ and the helper phage. When conditions comparable to those used in transformation studies were employed in transduction experiments, including the use of helper phage, two classes of transductants with either cys or gal linkage were also observed. To examine whether the location of the prophage on the E. coli chromosome had any effect on the ability of the prophage-associated tryp A+ and tryp B+ genes to function or respond to different repression conditions, specific activities of the A and B subunits of tryptophan synthetase specified by the phage genome were measured. Similar values were obtained regardless of the location of the prophage-associated tryp genes. Furthermore, the prophage-associated tryp genes, free from their normal operator region, permitted enzyme formation which was unaffected by repression or derepression conditions.

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Selected References

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  1. Calef E., Marchelli C., Guerrini F. The formation of superinfection-double lysogens of phage lambda in Escherichia coli K-12. Virology. 1965 Sep;27(1):1–10. doi: 10.1016/0042-6822(65)90136-4. [DOI] [PubMed] [Google Scholar]
  2. DUSSOIX D., ARBER W. HOST SPECIFICITY OF DNA PRODUCED BY ESCHERICHIA COLI. IV. HOST SPECIFICITY OF INFECTIOUS DNA FROM BACTERIOPHAGE LAMBDA. J Mol Biol. 1965 Feb;11:238–246. doi: 10.1016/s0022-2836(65)80054-7. [DOI] [PubMed] [Google Scholar]
  3. DUSSOIX D., ARBER W. Host specificity of DNA produced by Escherichia coli. II. Control over acceptance of DNA from infecting phage lambda. J Mol Biol. 1962 Jul;5:37–49. doi: 10.1016/s0022-2836(62)80059-x. [DOI] [PubMed] [Google Scholar]
  4. Gibson M. I., Gibson F. Preliminary studies on the isolation and metabolism of an intermediate in aromatic biosynthesis: chorismic acid. Biochem J. 1964 Feb;90(2):248–256. doi: 10.1042/bj0900248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ito J., Crawford I. P. Regulation of the enzymes of the tryptophan pathway in Escherichia coli. Genetics. 1965 Dec;52(6):1303–1316. doi: 10.1093/genetics/52.6.1303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KAISER A. D., HOGNESS D. S. The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg. J Mol Biol. 1960 Dec;2:392–415. doi: 10.1016/s0022-2836(60)80050-2. [DOI] [PubMed] [Google Scholar]
  7. KAISER A. D., JACOB F. Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localization. Virology. 1957 Dec;4(3):509–521. doi: 10.1016/0042-6822(57)90083-1. [DOI] [PubMed] [Google Scholar]
  8. Kaiser A. D., Inman R. B. Cohesion and the biological activity of bacteriophage lambda DNA. J Mol Biol. 1965 Aug;13(1):78–91. doi: 10.1016/s0022-2836(65)80081-x. [DOI] [PubMed] [Google Scholar]
  9. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  10. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  11. MATSUSHIRO A., KIDA S., ITO J., SATO K., IMAMOTO F. The regulatory mechanism of enzyme synthesis in the tryptophan biosynthetic pathway of Escherichia coli K-12. Biochem Biophys Res Commun. 1962 Oct 17;9:204–207. doi: 10.1016/0006-291x(62)90058-x. [DOI] [PubMed] [Google Scholar]
  12. MATSUSHIRO A., SATO K., ITO J., KIDA S., IMAMOTO F. ON THE TRANSCRIPTION OF THE TRYTOPHAN OPERON IN ESCHERICHIA COLI. I. THE TRYPTOPHAN OPERATOR. J Mol Biol. 1965 Jan;11:54–63. doi: 10.1016/s0022-2836(65)80170-x. [DOI] [PubMed] [Google Scholar]
  13. RADDING C. M., KAISER A. D. GENE TRANSFER BY BROKEN MOLECULES OF LAMBDA-DNA: ACTIVITY OF THE LEFT HALF-MOLECULE. J Mol Biol. 1963 Sep;7:225–233. doi: 10.1016/s0022-2836(63)80002-9. [DOI] [PubMed] [Google Scholar]
  14. SOMERVILLE R. L., YANOFSKY C. ON THE TRANSLATION OF THE A GENE REGION OF TRYPTOPHAN MESSENGER RNA. J Mol Biol. 1964 Apr;8:616–619. doi: 10.1016/s0022-2836(64)80019-x. [DOI] [PubMed] [Google Scholar]
  15. SOMERVILLE R. L., YANOFSKY C. STUDIES ON THE REGULATION OF TRYPTOPHAN BIOSYNTHESIS IN ESCHERICHIA COLI. J Mol Biol. 1965 Apr;11:747–759. doi: 10.1016/s0022-2836(65)80032-8. [DOI] [PubMed] [Google Scholar]
  16. STRACK H. B., KAISER A. D. ON THE STRUCTURE OF THE ENDS OF LAMBADA DNA. J Mol Biol. 1965 May;12:36–49. doi: 10.1016/s0022-2836(65)80280-7. [DOI] [PubMed] [Google Scholar]
  17. SWANSTROM M., ADAMS M. H. Agar layer method for production of high titer phage stocks. Proc Soc Exp Biol Med. 1951 Nov;78(2):372–375. doi: 10.3181/00379727-78-19076. [DOI] [PubMed] [Google Scholar]
  18. Signer E. R. Attachment specificity of prophage lambda-dg. J Mol Biol. 1965 Dec;14(2):582–585. doi: 10.1016/s0022-2836(65)80206-6. [DOI] [PubMed] [Google Scholar]
  19. Signer E. R., Beckwith J. R., Brenner S. Mapping of suppressor loci in Escherichia coli. J Mol Biol. 1965 Nov;14(1):153–166. doi: 10.1016/s0022-2836(65)80237-6. [DOI] [PubMed] [Google Scholar]
  20. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  21. YANOFSKY C., LENNOX E. S. Transduction and recombination study of linkage relationships among the genes controlling tryptophan synthesis in Escherichia coli. Virology. 1959 Aug;8:425–447. doi: 10.1016/0042-6822(59)90046-7. [DOI] [PubMed] [Google Scholar]
  22. Yamagishi H., Nakamura K., Ozeki H. Cohesion occurring between DNA molecules of temperate phages phi 80 and lambda or phi 81. Biochem Biophys Res Commun. 1965 Sep 22;20(6):727–732. doi: 10.1016/0006-291x(65)90077-x. [DOI] [PubMed] [Google Scholar]

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