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. 1966 Apr;91(4):1477–1488. doi: 10.1128/jb.91.4.1477-1488.1966

Properties and Regulation of the β-d-Galactosidase in Shigella dysenteriae and in Escherichia coli-Shigella dysenteriae Hybrids

S Sarkar a,1
PMCID: PMC316066  PMID: 5326112

Abstract

Sarkar, S. (Massachusetts Institute of Technology, Cambridge). Properties and regulation of the β-d-galactosidase in Shigella dysenteriae and in Escherichia coli-Shigella dysenteriae hybrids. J. Bacteriol. 91:1477–1488. 1966.—Shigella dysenteriae strain 60 has a β-d-galactosidase related to that of Escherichia coli but more heat-sensitive and with a turnover number about 10 times lower. Hybridization by transduction produces strains with enzymes of intermediate properties by recombination within the z gene. Both E. coli and S. dysenteriae have a regulatory i+ gene. Recombination between i− mutants of the two organisms leads to restoration of the i+ genotype. In S. dysenteriae 60, most of the i− mutants are subject to genetic suppression by suppressor mutations at unlinked loci. The effect of these suppressors on the products of the suppressed i− genes is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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