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. 1966 Apr;91(4):1548–1555. doi: 10.1128/jb.91.4.1548-1555.1966

Nonabsorbable Rabbit Anti-Salmonella typhimurium Antibody as Detected by the Complement-Mediated Bactericidal Reaction

Mendel Herzberg 1,2, Kathryn V Kenny 1,2, John B Robbins 1,2
PMCID: PMC316076  PMID: 4160234

Abstract

Herzberg, Mendel (University of Florida, Gainesville), Kathryn V. Kenny, and John B. Robbins. Nonabsorbable rabbit anti-Salmonella typhimurium antibody as detected by the complement-mediated bactericidal reaction. J. Bacteriol. 91:1548–1555. 1966.—A portion of antibody active in the complement-mediated bactericidal reaction against Salmonella typhimurium from hyperimmune rabbit serum has been shown to be nonabsorbable by repeated serial absorptions with whole heat-killed or living bacteria. The first two absorptions remove 90 to 95% of the activity, but 1 to 5% cannot be removed by subsequent absorptions. The nonabsorbable antibody appears to be a macroglobulin by density-gradient centrifugation and by comparison of activity and absorbability of purified γ-M and γ-G immune globulins. Alternative hypotheses involving low avidity antibody or antibody to minor cell antigenic components are offered in explanation of the phenomenon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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