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. 1966 Apr;91(4):1589–1593. doi: 10.1128/jb.91.4.1589-1593.1966

Quantitative Aspects of Exchangeable Calcium in Spores of Bacillus megaterium

L J Rode 1, J W Foster 1
PMCID: PMC316082  PMID: 4956344

Abstract

Rode, L. J. (The University of Texas, Austin), and J. W. Foster. Quantitative aspects of exchangeable calcium in spores of Bacillus megaterium. J. Bacteriol. 91:1589–1593. 1966.—More than 90% of the calcium in Ca45-labeled native spores was released from the cells during germination. Some 95% of the spore calcium was not exchangeable when ungerminated native spores were titrated to pH 4 with HNO3. Ca, Mg, Na, Si, and Fe were displaced from the spores by H+. The adsorption of Ca45 by H-spores and its subsequent release were studied under a variety of conditions. The isolated “coat fraction” of spores adsorbed substantial amounts of Ca45. Release of the adsorbed Ca was achieved with various reagents.

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Selected References

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  1. ALDERTON G., SNELL N. Base exchange and heat resistance in bacterial spores. Biochem Biophys Res Commun. 1963 Jan 31;10:139–143. doi: 10.1016/0006-291x(63)90039-1. [DOI] [PubMed] [Google Scholar]
  2. ALDERTON G., THOMPSON P. A., SNELL N. HEAT ADAPTATION AND ION EXCHANGE IN BACILLUS MEGATERIUM SPORES. Science. 1964 Jan 10;143(3602):141–143. doi: 10.1126/science.143.3602.141. [DOI] [PubMed] [Google Scholar]
  3. Foerster H. F., Foster J. W. Endotrophic calcium, strontium, and barium spores of Bacillus megaterium and Bacillus cereus. J Bacteriol. 1966 Mar;91(3):1333–1345. doi: 10.1128/jb.91.3.1333-1345.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KEYNAN A., HALVORSON H. O. Calcium dipicolinic acid-induced germination of Bacillus cereus spores. J Bacteriol. 1962 Jan;83:100–105. doi: 10.1128/jb.83.1.100-105.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. POWELL J. F. Factors affecting the germination of thick suspensions of bacillus subtilis spores in L-alanine solution. J Gen Microbiol. 1950 Sep;4(3):330–338. doi: 10.1099/00221287-4-3-330. [DOI] [PubMed] [Google Scholar]
  6. RODE L. J., FOSTER J. W. Germination of bacterial spores with alkyl primary amines. J Bacteriol. 1961 May;81:768–779. doi: 10.1128/jb.81.5.768-779.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. RODE L. J., FOSTER J. W. Ionic and non-ionic compounds in the germination of spores of Bacillus megaterium Texas. Arch Mikrobiol. 1962;43:201–212. doi: 10.1007/BF00406436. [DOI] [PubMed] [Google Scholar]
  8. Rode L. J., Foster J. W. Influence of exchangeable ions on germinability of bacterial spores. J Bacteriol. 1966 Apr;91(4):1582–1588. doi: 10.1128/jb.91.4.1582-1588.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rode L. J., Foster J. W. MECHANICAL GERMINATION OF BACTERIAL SPORES. Proc Natl Acad Sci U S A. 1960 Jan;46(1):118–128. doi: 10.1073/pnas.46.1.118. [DOI] [PMC free article] [PubMed] [Google Scholar]

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