Abstract
Nester, E. W. (University of Washington, Seattle), and R. A. Jensen. Control of aromatic acid biosynthesis in Bacillus subtilis: sequential feedback inhibition. J. Bacteriol. 91:1594–1598. 1966.—The three major end products of aromatic acid synthesis, tyrosine, phenylalanine, and tryptophan, were tested for their ability to inhibit the first enzymes of the three terminal branches of the pathway as well as the enzyme common to both tyrosine and phenylalanine synthesis. Tyrosine inhibits the activity of prephenate dehydrogenase and also prephenate dehydratase to a limited extent. Phenylalanine inhibits the activity of prephenate dehydratase and, at much higher concentrations, prephenate dehydrogenase. Tryptophan inhibits the activity of anthranilate synthetase and, to some extent, prephenate dehydrogenase and prephenate dehydratase. Chorismate mutase is not inhibited by either 1 mm tyrosine or 1 mm phenylalanine when these are present singly or together in the reaction mixture. The significance of the feedback control of the terminal branches to the feedback control of that part of the pathway common to the synthesis of all three amino acids is discussed.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AMES B. N., MARTIN R. G., GARRY B. J. The first step of histidine biosynthesis. J Biol Chem. 1961 Jul;236:2019–2026. [PubMed] [Google Scholar]
- COTTON R. G., GIBSON F. THE BIOSYNTHESIS OF PHENYLALANINE AND TYROSINE; ENZYMES CONVERTING CHORISMIC ACID INTO PREPHENIC ACID AND THEIR RELATIONSHIPS TO PREPHENATE DEHYDRATASE AND PREPHENATE DEHYDROGENASE. Biochim Biophys Acta. 1965 Apr 12;100:76–88. doi: 10.1016/0304-4165(65)90429-0. [DOI] [PubMed] [Google Scholar]
- Gibson F. Chorismic acid: purification and some chemical and physical studies. Biochem J. 1964 Feb;90(2):256–261. doi: 10.1042/bj0900256. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JENSEN R. A., NESTER E. W. THE REGULATORY SIGNIFICANCE OF INTERMEDIARY METABOLITES: CONTROL OF AROMATIC ACID BIOSYNTHESIS BY FEEDBACK INHIBITION IN BACILLUS SUBTILIS. J Mol Biol. 1965 Jun;12:468–481. doi: 10.1016/s0022-2836(65)80270-4. [DOI] [PubMed] [Google Scholar]
- LIN E. C., PITT B. M., CIVEN M., KNOX W. E. The assay of aromatic amino acid transaminations and keto acid oxidation by the enol borate-tautomerase method. J Biol Chem. 1958 Sep;233(3):668–673. [PubMed] [Google Scholar]
- METZENBERG R. L., MITCHELL H. K. Isolation of prephenic acid from Neurospora. Arch Biochem Biophys. 1956 Sep;64(1):51–56. doi: 10.1016/0003-9861(56)90240-5. [DOI] [PubMed] [Google Scholar]
- MOYED H. S. Interference with feedback control of enzyme activity. Cold Spring Harb Symp Quant Biol. 1961;26:323–329. doi: 10.1101/sqb.1961.026.01.039. [DOI] [PubMed] [Google Scholar]
- SCHWINCK I., ADAMS E. Aromatic biosynthesis. XVI. Aromatization of prephenic acid to p-hydroxyphenylpyruvic acid, a step in tyrosine biosynthesis in Escherichia coli. Biochim Biophys Acta. 1959 Nov;36:102–117. doi: 10.1016/0006-3002(59)90074-5. [DOI] [PubMed] [Google Scholar]
- STADTMAN E. R. Symptosium on multiple forms of enzymes and control mechanisms. II. Enzyme multiplicity and function in the regulation of divergent metabolic pathways. Bacteriol Rev. 1963 Jun;27:170–181. doi: 10.1128/br.27.2.170-181.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STURANE E., DATTA P., HUGHES M., GEST H. REGULATION OF ENZYME ACTIVITY BY SPECIFIC REVERSAL OF FEEDBACK INHIBITION. Science. 1963 Sep 13;141(3585):1053–1054. doi: 10.1126/science.141.3585.1053. [DOI] [PubMed] [Google Scholar]
- Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Umbarger H. E. Intracellular Regulatory Mechanisms: Regulation in multicellular forms may be an elaboration upon the pattern evolved in microorganisms. Science. 1964 Aug 14;145(3633):674–679. doi: 10.1126/science.145.3633.674. [DOI] [PubMed] [Google Scholar]