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. 1966 May;91(5):1763–1766. doi: 10.1128/jb.91.5.1763-1766.1966

Chromosomal Location of the Structural Gene for Glycerol Kinase in Escherichia coli

N R Cozzarelli 1, E C C Lin 1
PMCID: PMC316119  PMID: 5327904

Abstract

Cozzarelli, N. R. (Harvard Medical School, Boston, Mass.), and E. C. C. Lin. Chromosomal location of the structural gene for glycerol kinase in Escherichia coli. J. Bacteriol. 91:1763–1766. 1966.—A glycerol kinase mutant site has been mapped by transduction and sexual conjugation. Three-factor crosses with the two procedures yielded the following gene order: arginine-1–methionine-1–glycerol kinase–isoleucine, valine-16. An additional 13 independent glycerol kinase mutant sites mapped in the same region. Since some of the mutants were able to produce a protein serologically indistinguishable from the wild-type enzyme, it is concluded that the region mapped represents the structural gene for the kinase.

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Selected References

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