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. 1966 May;91(5):1787–1794. doi: 10.1128/jb.91.5.1787-1794.1966

Drug Resistance of Enteric Bacteria VI. Introduction of Bacteriophage P1CM into Salmonella typhi and Formation of P1dCM and F-CM Elements

Eiko Kondo 1, Susumu Mitsuhashi 1
PMCID: PMC316124  PMID: 5327907

Abstract

Kondo, Eiko (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Drug resistance of enteric bacteria. VI. Introduction of bacteriophage P1CM into Salmonella typhi and formation of P1dCM and F-CM elements. J. Bacteriol. 91:1787–1794. 1966.—Bacteriophage P1CM was introduced into Salmonella typhi by means of both phage infection and conjugation with Escherichia coli F+ lysogenic for the phage. Upon incubation with a P1CM phage lysate, S. typhi and S. abony yield CMr cells which are lysogenic for P1CM, but S. typhimurium LT2 does not. The P1CM phage is adsorbed slightly to S. typhi, but no infectious centers are formed when the phage is plated on this strain. Tests on P1CM-adsorbing capacity of the S. typhi P1CM+ strain and on plaque formation and transduction ability of the recovered phage from this strain indicated that the cell and the phage population did not have any special advantage over the original cell and phage population. Conjugation of S. typhi with E. coli F+ carrying P1CM+ gave three types of S. typhi CMr clones: those which carry the whole P1CM phage, those with the P1dCM element, and those with nontransferable CMr. The second type has the F factor and is sensitive to f phages in spite of its typical behavior, serologically and biochemically, as S. typhi. It can donate the P1dCM and F+ characters to E. coli F− or F−/P1 strains. As a consequence of conjugation with the E. coli F+ strain, the CMr character of the third type of S. typhi, the nontransferable CMr element, acquired conjugational transferability, owing to the formation of the element, F-CM. This element can be transferred to an E. coli F− strain at a very high frequency (ca. 100). Both the F and CMr determinants are jointly transduced with P1 phage and are jointly eliminated by acridine dye treatment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARBER W., KELLENBERGER G., WEIGLE J. La défectuosité du phage lambda transducteur. Schweiz Z Pathol Bakteriol. 1957;20(5):659–665. [PubMed] [Google Scholar]
  2. BERTANI G. Lysogeny. Adv Virus Res. 1958;5:151–193. doi: 10.1016/s0065-3527(08)60673-9. [DOI] [PubMed] [Google Scholar]
  3. BERTANI G., NICE S. J. Studies on lysogenesis. II. The effect of temperature on the lysogenization of Shigella dysenteriae with phage P1. J Bacteriol. 1954 Feb;67(2):202–209. doi: 10.1128/jb.67.2.202-209.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CAMPBELL A. Transduction and segregation in Escherichia coli K12. Virology. 1957 Oct;4(2):366–384. doi: 10.1016/0042-6822(57)90070-3. [DOI] [PubMed] [Google Scholar]
  5. Falkow S., Baron L. S. EPISOMIC ELEMENT IN A STRAIN OF SALMONELLA TYPHOSA. J Bacteriol. 1962 Sep;84(3):581–589. doi: 10.1128/jb.84.3.581-589.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HARADA K., KAMEDA M., SUZUKI M., MITSUHASHI S. DRUG RESISTANCE OF ENTERIC BACTERIA. 3. ACQUISITION OF TRANSFERABILITY OF NONTRANSMISSIBLE R(TC) FACTOR IN COOPERATION WITH F FACTOR AND FORMATION OF FR(TC). J Bacteriol. 1964 Nov;88:1257–1265. doi: 10.1128/jb.88.5.1257-1265.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HARADA K., SUZUKI M., KAMEDA M., MITSUHASHI S. On the drug-resistance of enteric bacteria. 2) Transmission of the drug-resistance among Enterobacteriaceae. Jpn J Exp Med. 1960 Aug;30:289–299. [PubMed] [Google Scholar]
  8. Hirota Y. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):57–64. doi: 10.1073/pnas.46.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. JOHNSON E. M., FALKOW S., BARON L. S. RECIPIENT ABILITY OF SALMONELLA TYPHOSA IN GENETIC CROSSES WITH ESCHERICHIA COLI. J Bacteriol. 1964 Jan;87:54–60. doi: 10.1128/jb.87.1.54-60.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KONDO E., HARADA K., MITSUHASHI S. Drug-resistance of enteric bacteria. 12. Transduction of the transmissible drug resistance factor by bacteriophage Plkc. Jpn J Exp Med. 1962 Feb;32:139–147. [PubMed] [Google Scholar]
  11. KONDO E., MITSUHASHI S. DRUG RESISTANCE OF ENTERIC BACTERIA. IV. ACTIVE TRANSDUCING BACTERIOPHAGE P1 CM PRODUCED BY THE COMBINATION OF R FACTOR WITH BACTERIOPHAGE P1. J Bacteriol. 1964 Nov;88:1266–1276. doi: 10.1128/jb.88.5.1266-1276.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kameda M., Harada K., Suzuki M., Mitsuhashi S. Drug resistance of enteric bacteria. V. High frequency of transduction of R factors with bacteriophage epsilon. J Bacteriol. 1965 Nov;90(5):1174–1181. doi: 10.1128/jb.90.5.1174-1181.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LOEB T. Isolation of a bacteriophage specific for the F plus and Hfr mating types of Escherichia coli K-12. Science. 1960 Mar 25;131(3404):932–933. doi: 10.1126/science.131.3404.932. [DOI] [PubMed] [Google Scholar]
  14. LOEB T., ZINDER N. D. A bacteriophage containing RNA. Proc Natl Acad Sci U S A. 1961 Mar 15;47:282–289. doi: 10.1073/pnas.47.3.282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LURIA S. E., ADAMS J. N., TING R. C. Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles. Virology. 1960 Nov;12:348–390. doi: 10.1016/0042-6822(60)90161-6. [DOI] [PubMed] [Google Scholar]
  16. LURIA S. E. Host-induced modifications of viruses. Cold Spring Harb Symp Quant Biol. 1953;18:237–244. doi: 10.1101/sqb.1953.018.01.034. [DOI] [PubMed] [Google Scholar]
  17. Lederberg J, Cavalli L L, Lederberg E M. Sex Compatibility in Escherichia Coli. Genetics. 1952 Nov;37(6):720–730. doi: 10.1093/genetics/37.6.720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Luria S E. Mutations of Bacterial Viruses Affecting Their Host Range. Genetics. 1945 Jan;30(1):84–99. doi: 10.1093/genetics/30.1.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. MATSUSHIRO A. Specialized transduction of tryptophan markers in Escherichia coli K12 by bacteriophage phi-80. Virology. 1963 Apr;19:475–482. doi: 10.1016/0042-6822(63)90041-2. [DOI] [PubMed] [Google Scholar]
  20. MITSUHASHI S., HARADA K., KAMEDA M. Elimination of transmissible drug-resistance by treatment with acriflavin. Nature. 1961 Mar 18;189:947–947. doi: 10.1038/189947a0. [DOI] [PubMed] [Google Scholar]
  21. MIYAKE T., DEMEREC M. Salmonella Escherichia hybrids. Nature. 1959 Jun 6;183(4675):1586–1586. doi: 10.1038/1831586a0. [DOI] [PubMed] [Google Scholar]
  22. Miyake T. Exchange of Genetic Material between Salmonella Typhimurium and Escherichia Coli K-12. Genetics. 1962 Aug;47(8):1043–1052. doi: 10.1093/genetics/47.8.1043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Morse M L, Lederberg E M, Lederberg J. Transduction in Escherichia Coli K-12. Genetics. 1956 Jan;41(1):142–156. doi: 10.1093/genetics/41.1.142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. ORSKOV I., ORSKOV F. An antigen termed f-plus occurring in F-plus E. coli strains. Acta Pathol Microbiol Scand. 1960;48:37–46. [PubMed] [Google Scholar]
  25. SWANSTROM M., ADAMS M. H. Agar layer method for production of high titer phage stocks. Proc Soc Exp Biol Med. 1951 Nov;78(2):372–375. doi: 10.3181/00379727-78-19076. [DOI] [PubMed] [Google Scholar]
  26. ZINDER N. D. Hybrids of Escherichia and Salmonella. Science. 1960 Mar 18;131(3403):813–815. doi: 10.1126/science.131.3403.813. [DOI] [PubMed] [Google Scholar]
  27. ZINDER N. D. Sexuality and mating in salmonella. Science. 1960 Mar 25;131(3404):924–926. doi: 10.1126/science.131.3404.924. [DOI] [PubMed] [Google Scholar]

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