Abstract
Yohn, David S. (Roswell Park Memorial Institute, Buffalo, N.Y.), Fanny R. Marmol, Victoria A. Haendiges, and James T. Grace, Jr. Yaba tumor poxvirus synthesis in vitro. II. Adsorption, inactivation, and assay studies. J. Bacteriol. 91:1953–1958. 1966.—Means to increase the efficiency of the Yaba tumor poxvirus assay in BSC-1 cell cultures were sought. A method was devised wherein 0.4 ml of each virus dilution was layered onto BSC-1 cells in 80-mm Leighton tubes and permitted to adsorb at 25 C for 18 hr prior to incubation at 35 C. The diluent found most reliable was medium 199 containing 50% bovine amniotic fluid adjusted to 2.0 mm calcium and 1.0 mm magnesium at pH 7.0. These modifications yielded highly reproducible titrations with a greater than twofold increase in assay sensitivity. Irreversible adsorption of Yaba virus by BSC-1 cells proceeds comparatively slowly at 25 to 33 C. Although the process is more rapid at 35 or 37 C, the increased thermal lability of the virus at these temperatures results in lower titers than with virus adsorbed at 25 C for longer periods of time. Yaba poxvirus appears to be 5 to 10 times more sensitive to thermal inactivation than vaccinia poxvirus.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dales S. Pentration of animal viruses into cells. Prog Med Virol. 1965;7:1–43. [PubMed] [Google Scholar]
- HIATT C. W. KINETICS OF THE INACTIVATION OF VIRUSES. Bacteriol Rev. 1964 Jun;28:150–163. doi: 10.1128/br.28.2.150-163.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KAPLAN C. The heat inactivation of vaccinia virus. J Gen Microbiol. 1958 Feb;18(1):58–63. doi: 10.1099/00221287-18-1-58. [DOI] [PubMed] [Google Scholar]
- YOHN D. S., GRACE J. T., Jr, HAENDIGES V. A. A QUANTITATIVE CELL CULTURE ASSAY FOR YABA TUMOUR VIRUS. Nature. 1964 May 30;202:881–883. doi: 10.1038/202881a0. [DOI] [PubMed] [Google Scholar]
- Yohn D. S., Haendiges V. A., De Harven E. Yaba tumor poxvirus synthesis in vitro. 3. Growth kinetics. J Bacteriol. 1966 May;91(5):1986–1991. doi: 10.1128/jb.91.5.1986-1991.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yohn D. S., Haendiges V. A., Grace J. T., Jr Yaba tumor poxvirus synthesis in vitro. I. Cytopathological, histochemical, and immunofluorescent studies. J Bacteriol. 1966 May;91(5):1977–1985. doi: 10.1128/jb.91.5.1977-1985.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]