Abstract
Schaffer, Frederick L. (University of California, Berkeley), and Marjorie Gordon. Differential inhibitory effects of actinomycin D among strains of poliovirus. J. Bacteriol. 91:2309–2316. 1966.—Actinomycin D exerted a differential effect on the ability of strains of poliovirus to replicate in HeLa cells. LSc-2ab was studied as an example of a strain markedly inhibited by actinomycin; MEF1 served as a control strain with minimal inhibition. The effect was noted at an actinomycin concentration of 0.1 μg/ml, but 2.5 μg/ml was used for most studies. Variability in the effect was attributed, in part, to physiological factors. Actinomycin was effective when present during the first 2 hr of LSc infection, but had little effect if present at later times. It did not block adsorption or initiation of ecilpse. It did block synthesis of ribonucleic acid in LSc-infected cells. Several possible modes of action are discussed, the most attractive being that actinomycin blocks synthesis of some cell component, the concentration of which is more critical for replication of some poliovirus strains than others.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BADER J. P. THE REQUIREMENT FOR DNA SYNTHESIS IN THE GROWTH OF ROUS SARCOMA AND ROUS-ASSOCIATED VIRUSES. Virology. 1965 Jun;26:253–261. doi: 10.1016/0042-6822(65)90272-2. [DOI] [PubMed] [Google Scholar]
- BUKRINSKAYA A. G. RESISTANCE OF SYNTHESIS OF INFLUENZA VIRUS RIBONUCLEIC ACID TO THE ACTION OF ACTINOMYCIN D. Nature. 1964 Oct 10;204:205–206. doi: 10.1038/204205a0. [DOI] [PubMed] [Google Scholar]
- GOMATOS P. J., KRUG R. M., TAMM I. ENZYMIC SYNTHESIS OF RNA WITH REOVIRUS RNA AS TEMPLATE. I. CHARACTERISTICS OF THE REACTION CATALYZED BY THE RNA POLYMERASE FROM ESCHERICHIA COLI. J Mol Biol. 1964 Jul;9:193–207. doi: 10.1016/s0022-2836(64)80100-5. [DOI] [PubMed] [Google Scholar]
- Grado C., Fischer S., Contreras G. The inhibition by actinomycin D of poliovirus multiplication HEp 2 cells. Virology. 1965 Dec;27(4):623–625. doi: 10.1016/0042-6822(65)90189-3. [DOI] [PubMed] [Google Scholar]
- HAUSEN P., VERWOERD D. W. STUDIES ON THE MULTIPLICATION OF A MEMBER OF THE COLUMBIA SK GROUP (ME VIRUS) IN L CELLS. III. ALTERATION OF RNA AND PROTEIN SYNTHETIC PATTERNS IN VIRUS-INFECTED CELLS. Virology. 1963 Dec;21:617–627. doi: 10.1016/0042-6822(63)90235-6. [DOI] [PubMed] [Google Scholar]
- HOLLAND J. J., HOYER B. H., McLAREN L. C., SYVERTON J. T. Enteroviral ribonucleic acid. I. Recovery from virus and assimilation by cells. J Exp Med. 1960 Nov 1;112:821–839. doi: 10.1084/jem.112.5.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOMMA M., GRAHAM A. F. SYNTHESIS OF RNA IN L CELLS INFECTED WITH MENGO VIRUS. J Cell Physiol. 1963 Oct;62:179–192. doi: 10.1002/jcp.1030620207. [DOI] [PubMed] [Google Scholar]
- Honig G. R., Rabinovitz M. Actinomycin D: inhibition of protein synthesis unrelated to effect on template RNA synthesis. Science. 1965 Sep 24;149(3691):1504–1506. doi: 10.1126/science.149.3691.1504. [DOI] [PubMed] [Google Scholar]
- KORN D., PROTASS J. J., LEIVE L. A NOVEL EFFECT OF ACTINOMYCIN D IN PREVENTING BACTERIOPHAGE T4 MATURATION IN ESCHERICHIA COLI. Biochem Biophys Res Commun. 1965 May 3;19:473–481. doi: 10.1016/0006-291x(65)90149-x. [DOI] [PubMed] [Google Scholar]
- MANDEL B. The use of sodium dodecyl sulfate in studies on the interaction of poliovirus and HeLa cells. Virology. 1962 Jun;17:288–294. doi: 10.1016/0042-6822(62)90119-8. [DOI] [PubMed] [Google Scholar]
- REICH E. BIOCHEMISTRY OF ACTINOMYCINS. Cancer Res. 1963 Sep;23:1428–1441. [PubMed] [Google Scholar]
- REICH E., FRANKLIN R. M., SHATKIN A. J., TATUMEL Action of actinomycin D on animal cells and viruses. Proc Natl Acad Sci U S A. 1962 Jul 15;48:1238–1245. doi: 10.1073/pnas.48.7.1238. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REVEL M., HIATT H. H., REVEL J. P. ACTINOMYCIN D: AN EFFECT ON RAT LIVER HOMOGENATES UNRELATED TO ITS ACTION ON RNA SYNTHESIS. Science. 1964 Dec 4;146(3649):1311–1313. doi: 10.1126/science.146.3649.1311. [DOI] [PubMed] [Google Scholar]
- ROTT R., SCHOLTISSEK C. EINFLUSS VON ACTINOMYCIN AUF DIE VERMEHRUNG VON MYXOVIREN. Z Naturforsch B. 1964 Apr;19:316–323. doi: 10.1007/BF01191316. [DOI] [PubMed] [Google Scholar]
- Reich E., Goldberg I. H. Actinomycin and nucleic acid function. Prog Nucleic Acid Res Mol Biol. 1964;3:183–234. doi: 10.1016/s0079-6603(08)60742-4. [DOI] [PubMed] [Google Scholar]
- SCHAFFER F. L. Binding of proflavine by and photoinactivation of poliovirus propagated in the presence of the dye. Virology. 1962 Nov;18:412–425. doi: 10.1016/0042-6822(62)90032-6. [DOI] [PubMed] [Google Scholar]
- SHATKIN A. J. ACTINOMYCIN AND THE DIFFERENTIAL SYNTHESIS OF REOVIRUS AND L CELL RNA. Biochem Biophys Res Commun. 1965 May 3;19:506–510. doi: 10.1016/0006-291x(65)90154-3. [DOI] [PubMed] [Google Scholar]
- SPENDLOVE R. S., TAYLOR F. L. A rack for expediting the manipulation of tissue culture bottles, with an application to the viral plaque technic. Am J Clin Pathol. 1963 Jul;40:34–37. doi: 10.1093/ajcp/40.1.34. [DOI] [PubMed] [Google Scholar]
- TAKEMOTO K. K., LIEBHABER H. Virus-polysaccharide interactions. II. Enhancement of plague formation and the detection of variants of poliovirus with dextran sulfate. Virology. 1962 Jul;17:499–501. doi: 10.1016/0042-6822(62)90148-4. [DOI] [PubMed] [Google Scholar]
- TEMIN H. M. THE PARTICIPATION OF DNA IN ROUS SARCOMA VIRUS PRODUCTION. Virology. 1964 Aug;23:486–494. doi: 10.1016/0042-6822(64)90232-6. [DOI] [PubMed] [Google Scholar]
- VOGT M., DULBECCO R., WENNER H. A. Mutants of poliomyelitis viruses with reduced efficiency of plating in acid medium and reduced neuropathogenicity. Virology. 1957 Aug;4(1):141–155. doi: 10.1016/0042-6822(57)90050-8. [DOI] [PubMed] [Google Scholar]
- WHITE D. O., DAY H. M., BATCHELDER E. J., CHEYNE I. M., WANSBROUGH A. J. DELAY IN THE MULTIPLICATION OF INFLUENZA VIRUS. Virology. 1965 Feb;25:289–302. doi: 10.1016/0042-6822(65)90207-2. [DOI] [PubMed] [Google Scholar]
- Zhdanov V. M., Yershov F. I. Photodynamic and fluorochromic properties of the antibiotic actinomycin D. J Cell Physiol. 1965 Jun;65(3):433–434. doi: 10.1002/jcp.1030650317. [DOI] [PubMed] [Google Scholar]