Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Journal of Bacteriology logoLink to Journal of Bacteriology
. 1966 Jun;91(6):2368–2376. doi: 10.1128/jb.91.6.2368-2376.1966

Fluorescent-Antibody and Histological Study of Vaccinated and Control Monkeys Challenged with Shigella flexneri

Samuel B Formal 1, T H Kent 1, S Austin 1, E H LaBrec 1
PMCID: PMC316221  PMID: 4957617

Abstract

Formal, Samuel B., (Walter Reed Army Institute of Research, Washington, D.C.), T. H. Kent, S. Austin, and E. H. LaBrec. Fluorescent-antibody and histological study of vaccinated and control monkeys challenged with Shigella flexneri. J. Bacteriol. 91:2368–2376. 1966.—Groups of monkeys were fed four doses of a living Escherichia coli-Shigella flexneri 2a hybrid strain, and, together with control animals, were challenged with virulent S. flexneri 2a. Two experiments were carried out; in the first, the animals were challenged 10 days after and in the second, 1 month after the last vaccine dose was administered. At 48 hr after challenge, tissues were removed from the vaccinated and control animals, and examined by use of histological and fluorescent-antibody techniques. The results of this study demonstrate that the animals receiving the vaccine were protected from the tissue damage ordinarily observed after experimental challenge with virulent dysentery bacilli. The virulent challenge strain appeared to be unable to penetrate into the intestinal mucosa of immunized animals.

Full text

PDF
2368

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. FORMAL S. B., LABREC E. H., KENT T. H., FALKOW S. ABORTIVE INTESTINAL INFECTION WITH AN ESCHERICHIA COLI-SHIGELLA FLEXNERI HYBRID STRAIN. J Bacteriol. 1965 May;89:1374–1382. doi: 10.1128/jb.89.5.1374-1382.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Formal S. B., Labrec E. H., Palmer A., Falkow S. Protection of Monkeys Against Experimental Shigellosis with Attenuated Vaccines. J Bacteriol. 1965 Jul;90(1):63–68. doi: 10.1128/jb.90.1.63-68.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. LABREC E. H., FORMAL S. B. Experimental Shigella infections. IV. Fluorescent antibody studies of an infection in guinea pigs. J Immunol. 1961 Nov;87:562–572. [PubMed] [Google Scholar]
  4. Labrec E. H., Schneider H., Magnani T. J., Formal S. B. EPITHELIAL CELL PENETRATION AS AN ESSENTIAL STEP IN THE PATHOGENESIS OF BACILLARY DYSENTERY. J Bacteriol. 1964 Nov;88(5):1503–1518. doi: 10.1128/jb.88.5.1503-1518.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES