Abstract
Shieh, K. Z. (Illinois Institute of Technology, Chicago), and L. R. Hedrick. Energy requirement for l-glutamate uptake and utilization by Hansenula subpelliculosa cells. J. Bacteriol. 92:1638–1644. 1966.—Cells of the yeast Hansenula subpelliculosa require an energy source for the uptake of glutamate. A lag period of 20 to 40 min was required after the addition of glucose to the cells before glutamate uptake was initiated. When cells were preincubated in glucose, and washed with distilled water prior to the addition of glutamate, there was no lag period. Preincubation in glucose and glutamate lowered both the rate and the total uptake of glutamate as compared with cells preincubated in glucose alone. This is attributed to the partial utilization of the glucose-metabolite by glutamate or to the partial saturation of binding sites by glutamate during the preincubation period. Transport of glutamate by these yeast cells appears to be via a carrier, where energy is required for the binding of the amino acid to nonspecific binding sites. In addition to total uptake, some aspects of the C14-glutamate utilization were measured. Of the total uptake, 58% was metabolized and converted to CO2, 25.2% remained in the soluble pool, and 16.8% was incorporated into trichloroacetic acid-insoluble products. When the available energy source was depleted, the processes of uptake, metabolism, and incorporation ceased, even though there was an ample supply of glutamate present within the cells. Removal of cells from glutamate and addition of glucose reinitiated the incorporation of glutamate into proteins and other trichloroacetic acid-insoluble compounds. Therefore, an additional energy source is required with this species of yeast for glutamate uptake, for the priming of mechanisms required for its metabolism, and for its incorporation.
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Selected References
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- BALAZS R. CONTROL OF GLUTAMATE OXIDATION IN BRAIN AND LIVER MITOCHONDRIAL SYSTEMS. Biochem J. 1965 May;95:497–508. doi: 10.1042/bj0950497. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BRITTEN R. J., McCLURE F. T. The amino acid pool in Escherichia coli. Bacteriol Rev. 1962 Sep;26:292–335. doi: 10.1128/br.26.3.292-335.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CONWAY E. J., DOWNEY M. An outer metabolic region of the yeast cell. Biochem J. 1950 Sep;47(3):347–355. doi: 10.1042/bj0470347. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ECKER R. E., LOCKHART W. R. Specific effect of limiting nutrient on physiological events during culture growth. J Bacteriol. 1961 Oct;82:511–516. doi: 10.1128/jb.82.4.511-516.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HALVORSON H. O., SPIEGELMAN S. The effect of free amino acid pool levels on the induced synthesis of enzymes. J Bacteriol. 1953 May;65(5):496–504. doi: 10.1128/jb.65.5.496-504.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HALVORSON H. O., SPIEGELMAN S. The inhibition of enzyme formation by amino acid analogues. J Bacteriol. 1952 Aug;64(2):207–221. doi: 10.1128/jb.64.2.207-221.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Halpern Y. S., Lupo M. Glutamate transport in wild-type and mutant strains of Escherichia coli. J Bacteriol. 1965 Nov;90(5):1288–1295. doi: 10.1128/jb.90.5.1288-1295.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LING C. M., HEDRICK L. R. PROLINE OXIDASES IN HANSENULA SUBPELLICULOSA. J Bacteriol. 1964 Jun;87:1462–1470. doi: 10.1128/jb.87.6.1462-1470.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pichler A. G. Some characteristics of the utilization of L-glutamic acid by Ehrlich ascites-tumor cells. Biochim Biophys Acta. 1965 Jun 15;104(1):104–111. doi: 10.1016/0304-4165(65)90226-6. [DOI] [PubMed] [Google Scholar]
- QUETSCH M. F., DANFORTH W. F. THE NATURE OF THE "ADAPTIVE LAG" IN URIC ACID ACCUMULATION BY THE YEAST, CANDIDA UTILIS. J Cell Physiol. 1964 Aug;64:115–121. doi: 10.1002/jcp.1030640111. [DOI] [PubMed] [Google Scholar]
- ROSENBERG L. E., WEINBERG A. N., SEGAL S. The effect of high galactose diets on urinary excretion of amino acids in the rat. Biochim Biophys Acta. 1961 Apr 15;48:500–505. doi: 10.1016/0006-3002(61)90047-6. [DOI] [PubMed] [Google Scholar]
- Vender J., Jayaraman K., Rickenberg H. V. Metabolism of glutamic acid in a mutant of Escherichia coli. J Bacteriol. 1965 Nov;90(5):1304–1307. doi: 10.1128/jb.90.5.1304-1307.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wickerham L. J. A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts. J Bacteriol. 1946 Sep;52(3):293–301. [PMC free article] [PubMed] [Google Scholar]