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. Author manuscript; available in PMC: 2012 Oct 10.
Published in final edited form as: Gene. 2011 Jun 30;485(2):130–138. doi: 10.1016/j.gene.2011.06.020

Fig. 4.

Fig. 4

Both the RRM and RS domains of SRp75 are required for inhibition of exon 10 splicing. (A) Diagram of SRp75 variants used in this study. The domains and the amino acids that code for them are indicated. Total length of hnRNPE3 is 495 amino acids. (B) The SRp75 variants express as FLAG fusion proteins in COS cells. The proteins on the Western blot were detected by anti-FLAG monoclonal antibody M2. Protein markers are indicated on the left of the panel. The cell lysates were normalized for protein content. (C) RT-PCR of SP/10L in COS cells in the presence of full-length and single-domain variants of SRp75. The RT-PCR products come from 1:1 co-transfections of SP/10L and FLAG- SRp75 fusion constructs. Exon ratio calculations, primers, graph conventions and p-value notations are as in Fig. 1C.