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. Author manuscript; available in PMC: 2012 Sep 1.

Published in final edited form as: Virology. 2011 Jul 14;417(2):327–333. doi: 10.1016/j.virol.2011.06.009

Fig. 2 — (a) Balb/c mice were inoculated by the intro-tracheal route with a single dose of 1×10¹¹vg/mouse in a volume of 50ul of AAV1-GFP (panels A, B and C) or AAV6-GFP (D, E, and F). Mouse trachea and lungs were harvested at 1 month p.i.. Paraffin-embedded tissue sections were prepared, and transduced cells were immunolabeled with rabbit anti-GFP antibody (Sigma) followed by Alexafluor594- conjugated goat anti-rabbit antibody (Invitrogen). Nuclei were stained blue with DAPI. Representative images for alveolar (A & D), bronchiolus (B & E), and tracheal regions (C & F) are shown. (b) The numbers of GFP positive cells per image field were quantified and the mean numbers from five random images were shown (± SEM).

Fig. 2 — (a) Balb/c mice were inoculated by the intro-tracheal route with a single dose of 1×10¹¹vg/mouse in a volume of 50ul of AAV1-GFP (panels A, B and C) or AAV6-GFP (D, E, and F). Mouse trachea and lungs were harvested at 1 month p.i.. Paraffin-embedded tissue sections were prepared, and transduced cells were immunolabeled with rabbit anti-GFP antibody (Sigma) followed by Alexafluor594- conjugated goat anti-rabbit antibody (Invitrogen). Nuclei were stained blue with DAPI. Representative images for alveolar (A & D), bronchiolus (B & E), and tracheal regions (C & F) are shown. (b) The numbers of GFP positive cells per image field were quantified and the mean numbers from five random images were shown (± SEM).