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. Author manuscript; available in PMC: 2012 Aug 3.
Published in final edited form as: Nanoscale. 2011 Jul 13;3(8):3395–3407. doi: 10.1039/c1nr10427f

Fig. 5.

Fig. 5

Tapping mode-AFM topographs and amplitude images showing the exterior of the NHS functionalized nanoporous gold surfaces after conjugation of BSA. Top panels represent the amplitude images; lower panels represent the topographic images of the same areas as in top panels. (A) 500 nm × 500 nm, NHS ester functionalized gold surface without exposure to BSA. Similarly, 500 nm × 500 nm, AFM topographic images of NHS ester functionalized gold surface after exposure to BSA (4 ml of BSA, PBS buffer pH 7.0, 24 h incubation at 4 °C), at (B) 1 μg ml−1, (C) 5 μg ml−1, (D) 10 μg ml−1 and (E) 20 μg ml−1.