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. Author manuscript; available in PMC: 2012 Sep 13.
Published in final edited form as: Biochemistry. 2011 Aug 17;50(36):7774–7786. doi: 10.1021/bi200847n

Figure 3. G6 treatment induces marked reorganization of vimentin intermediate filaments within cells.

Figure 3

HEL cells, treated with 25 μM of G6 for 0 or 24 h, were analyzed via indirect immunofluorescence for changes in the cellular distribution of vimentin and β-actin in response to drug treatment. Vimentin (A and B) and β-actin (G and H) were indirectly labeled with a FITC-conjugated secondary antibody. The nuclei were counter stained with DAPI (C, D and I, J). The images were then merged (E, F and K, L). Shown is one of two representative results.