Skip to main content
. Author manuscript; available in PMC: 2012 Sep 13.
Published in final edited form as: Biochemistry. 2011 Aug 17;50(36):7774–7786. doi: 10.1021/bi200847n

Figure 5. G6-induced cleavage of vimentin is independent of de novo protein synthesis and caspase activity, but calpain-dependent.

Figure 5

HEL cells were pretreated for 4 hours with either cycloheximide (CHX) (A), caspase inhibitor I (zVAD) (B) or calpain inhibitor V (C) and then treated with increasing doses of G6 for 24 hours. Whole cell lysates from the different treatment groups were then analyzed by western blotting with an anti-vimentin antibody. The same lysates were also probed with an anti-β-actin antibody to confirm equal protein loading across all lanes. Shown is one of three representative results for each.