TABLE 1.

HSV-2 peptide pools used in the IFN-γ ELISPOT assay

No.	HSV-2 ORF	ORF classa	Common name	ORF Length (aa)	# peptidesb	# pools	# peptides/pool
1	US6	gp	gD-2	393	96	1	96
2	RL2	IE	ICP0	824	198	2	98,100
3	US1	IE	ICP22	413	101	2	50,51
4	UL54	IE	ICP27	512	126	2	63,63
5	RS1	IE	ICP4	1,318	330	4	82,82,83,83
6	UL39	other	ICP6	1,142	283	3	94,94,95
7	UL19	capsid	VP5/ICP5	1,375	341	4	85,85,85,86
8	UL25	capsid		585	144	2	72,72
9	UL35	capsid	VP26	112	26	1	26
10	UL46	tegument	VP11/12	722	178	2	89,89
11	UL47	tegument	VP13/14	696	172	2	86,86
12	UL49	tegument	VP22	300	74	1	74
13	UL11	tegument		96	22	1	22
14	UL27	gp	gB	904	224	3	74,87,63
15	UL29	other	ICP8	1,196	297	3	99,99,99
16	US5	gp	gJ	92	21	1	21
Totals					2,633	34	range: 21–100

^aOpen reading frames (ORF) for study encoded glycoproteins (gp), immediate early (IE), capsid, tegument or other proteins.

^b15 mers overlapping by 11 amino acids (aa).