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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Allergy. 2011 May 24;66(10):1304–1311. doi: 10.1111/j.1398-9995.2011.02647.x

Figure 3.

Figure 3

LTB4 and LTD4 signal through Gαi/o-coupled BLT1 and p44/42 MAPK in monocytes. Monocytes were pretreated with pertussis toxin (100 ng/mL) overnight and then stimulated with LTB4 (50 nmol/L) or LTD4 (100 nmol/L) (A), or stimulated with LTB4 and LTD4 together (B). Monocytes were stimulated with LTB4 (C) or LTD4 (D) at different time points and phosphorylation of p44/42 was assayed by immunoblotting. (E) Cells were preincubated with CP105696 (10 nmol/L) for 20 min and stimulated with LTB4 for 1 or 2 min. (F) Cells were preincubated with pertussis toxin (100 ng/mL) overnight and stimulated with LTB4 (50 nmol/L), LTD4 (100 nmol/L) or LTB4 and LTD4 for 2 min. Data are representative of three independent experiments.