FDG-PET metabolic response predicts outcomes in anal cancer managed with chemoradiotherapy

Abstract

Background:

The aim was to investigate the correlation between ¹⁸F-fluorodeoxyglucose positron emission tomography (FDG-PET) metabolic response to chemoradiotherapy and clinical outcomes in squamous cell carcinoma (SCC) of the anus.

Methods:

A total of 48 patients with biopsy-proven anal SCC underwent FDG-PET scans at baseline and post chemoradiotherapy (54 Gy, concurrent 5-FU/mitomycin). Kaplan–Meier analysis was used to determine survival outcomes according to FDG-PET metabolic response.

Results:

In all, 79% patients (n=38) had a complete metabolic response (CMR) at all sites of disease, 15% (n=7) had a CMR in regional nodes but only partial response in the primary tumour (overall partial metabolic response (PMR)) and 6% (n=3) had progressive distant disease despite CMR locoregionally (overall no response (NR)). The 2-year progression-free survival (PFS) was 95% for patients with a CMR, 71% for PMR and 0% for NR (P<0.0001). The 5-year overall survival (OS) was 88% in CMR, 69% in PMR and 0% in NR (P<0.0001). Cox proportional hazards regression analyses for PFS and OS found significant associations for incomplete (PMR+NR) vs complete FDG-PET response to treatment only, (HR 4.1 (95% CI: 1.5–11.5, P=0.013) and 6.7 (95% CI: 2.1–21.6, P=0.002), respectively).

Conclusion:

FDG-PET metabolic response to chemoradiotherapy in anal cancer is significantly associated with PFS and OS, and in this cohort incomplete FDG-PET response was a stronger predictor than T or N stage.

Squamous cell carcinoma (SCC) of the anus is an uncommon malignancy, but increasing in incidence (Johnson et al, 2004; Robinson et al, 2009). Aetiologic factors include infection by human papilloma virus (Frisch et al, 1997) and/or human immunodeficiency virus (Palefsky et al, 1998) and smoking (Holly et al, 1989). Historically, anal cancer was managed surgically with abdominoperineal resection (APR), but since the landmark publication by Nigro et al, 1974, chemoradiotherapy has been the mainstay of treatment. This management approach allows preservation of the anal sphincter, avoidance of permanent colostomy and prophylactic radiotherapy to the regional nodal basins. The all-stage 5-year overall survival (OS) with current therapy is 58% (Bilimoria et al, 2009).

Staging of anal cancer by clinical assessment (primary and inguinal nodes) was later supplemented by the use of computed tomography (CT) (Scherrer et al, 1990) and, in some cases, additional anatomic imaging of the pelvis with magnetic resonance imaging or ultrasound. Multiple recent studies have now demonstrated the utility of ¹⁸F-fluorodeoxyglucose positron emission tomography (FDG-PET) in detecting inguinal or pelvic node involvement not evident clinically or on CT (Trautmann and Zuger, 2005; Cotter et al, 2006; Nguyen et al, 2008; Iagaru et al, 2009; de Winton et al, 2009), with subsequent impact on the planning of definitive radiotherapy fields (Nguyen et al, 2008; de Winton et al, 2009). The FDG-PET staging of anal cancer has become the standard of care in many centres.

Post-treatment FDG-PET assessment of tumour metabolic response is increasingly employed in treatment protocols for Hodgkin's and non-Hodgkin's lymphomas (Juweid et al, 2007), non-small cell lung cancer (MacManus et al, 2003), oesophageal (Duong et al, 2006) and head and neck cancers (Andrade et al, 2006). For some FDG-avid malignancies, FDG-PET has been demonstrated to be more accurate than CT alone in the evaluation of tumour response to therapy (MacManus et al, 2003; Andrade et al, 2006). Tumour metabolic response has also been demonstrated to provide robust prognostic information, particularly in lymphoma where both positive and negative predictive values for interim and post-treatment FDG-PET scans and disease progression are high (Mikhaeel et al, 2000; Kobe et al, 2008). Currently, assessment of response to chemoradiotherapy in anal carcinoma relies on serial clinical examination from 8 to 12 weeks post chemoradiotherapy (NCCN Clinical Practice Guidelines in Oncology, V.1.2010). However, clinical estimation of tumour response is subjective and may be confounded by radiotherapy-related skin toxicity (Borzomati et al, 2005) and residual nonmalignant masses (Sato et al, 2005). Histopathological interpretation of biopsies performed for nonresolving lesions or new in-field changes may also be difficult in the setting of post-radiotherapy change.

Our aim was to investigate the clinical significance of FDG-PET metabolic response to radical chemoradiotherapy in SCC of the anus. FDG-PET responses to treatment were evaluated and correlated with the end points of progression-free survival (PFS), OS and cause-specific survival. We also examined the prognostic power of post-therapy FDG-PET in comparison with the known anal cancer prognostic variables of baseline tumour T and N stage (Deniaud-Alexandre et al, 2003; Bilimoria et al, 2009; Myerson et al, 2009).

Materials and methods

Study population

Patients with a diagnosis of anal carcinoma were retrospectively identified from our institutions’ PET Centre electronic research database. Incorporation within the database signifies receipt of one or more PET scans, and provision of individual patient consent to data use for research purposes. Institutional ethics committee approval was granted for research applications of the database. Patient records were then manually reviewed to ensure histologic confirmation of anal carcinoma, planned radical chemoradiotherapy and performance of a baseline pretreatment FDG-PET scan. A total of 74 patients met these criteria between September 1997 and April 2006. The impact of baseline FDG-PET scan results on the staging and radiotherapy field planning of a proportion of these patients (61 patients, from September 1997 to November 2005) has been previously reported (de Winton et al, 2009). Patients within the cohort who additionally underwent post-chemoradiotherapy FDG-PET assessment of tumour response (n=48) were identified for this study and all included for analysis.

FDG-PET imaging and interpretation

Both pre- and post-treatment FDG imaging was with dedicated PET in 20 patients, hybrid PET-CT in 20 patients and one scan of each type in 8 patients. PET scans were acquired on a GE QUEST 300-H (3D mode, sodium iodide detector) scanner (UGM Medical Systems, Inc., Philadelphia, PA, USA) and PET-CT scans on a GE DLS PET-CT (2D mode, Bismuth Germanate detector) scanner (Discovery LS; GE Healthcare, Waukesha, WI, USA). The PET images were acquired at least 1 h after intravenous injection of 80–120 MBq of ¹⁸F-FDG on the GE Quest scanner and 300–400 MBq on the GE DLS scanner. All patients fasted for 6 h before the PET study but were encouraged to drink water. Patients were catheterised and administered furosemide 30 min before imaging to minimise bladder activity, and also received bowel preparation. The blood glucose level of the patients was required to be <10 mmol l^–1 before FDG administration.

Transmission and emission scans were obtained from the lower neck to the upper thighs. Emission data were processed using OSEM (ordered-subset expectation maximisation method) reconstruction. Attenuation correction with single transmission source (Benard et al, 1999) was performed on the GE Quest scanner and with CT data on the GE DLS scanner. Data sets were reported both with and without attenuation correction. Rotating count-rendered images were also reviewed to aid clarification of the relationship between the physiological radiotracer accumulation and tumour in the anus.

All PET and PET-CT studies were reported on the day of the scan by experienced PET specialists. Pretreatment PET images were available for unblinded review and comparison during post-treatment scan interpretation. For chest, abdominal and pelvic activity, abnormal focal uptake of ¹⁸F-FDG had to be greater than mediastinal uptake, and needed to correspond to an anatomical structure or abnormality identified on CT; for example, a lymph node of normal or abnormal size. Any ¹⁸F-FDG activity less than mediastinal blood pool activity was defined as abnormal only if there was a definite corresponding structural abnormality of <1 cm in size (because of the known partial volume effect of PET-CT caused by its limited resolution below 1 cm). Increased uptake in the radiotherapy field in a geographic distribution or not conforming to anatomical structures involved at baseline was assumed to represent post-radiotherapy changes as previously described (Kalff et al, 2009).

A complete metabolic response (CMR) to treatment was defined as a return of visually graded ¹⁸F-FDG uptake in all baseline lesions to a level equivalent to or lower than the radioactivity in normal tissues of the involved organ, as previously described (MacManus et al, 2003; Hicks, 2009). Partial metabolic response (PMR) was defined as an improvement in visually graded ¹⁸F-FDG uptake at baseline involved sites, but persistent residual abnormality suggesting malignancy, and additionally included equivocal scans where residual disease could not be excluded. No response (NR) to treatment was defined as no change or an increase in ¹⁸F-FDG uptake within a baseline lesion, consistent with tumour growth, or development of a new site of disease. The worst response at any site was used for categorisation.

Chemoradiotherapy

All patients received definitive external beam radiotherapy with concurrent chemotherapy. Radiotherapy was delivered using 6–18 MV photons to a total dose of 54 Gy in 1.8 Gy daily fractions, five fractions per week using a three-phase technique. Phase 1 consisted of anterior–posterior parallel opposed fields, with the clinical target volume (CTV) covering the primary tumour, perirectal, iliac and inguinal lymph nodes to a total dose of 36 Gy. Phase 2 consisted of a three-field technique, with the CTV encompassing the primary tumour and anal canal, perirectal and iliac nodes to a total dose of 45 Gy. Phase 3 consisted of a reduced three-field technique that boosted the primary tumour and anal canal to a total dose of 54 Gy. Any involved inguinal or pelvic nodes were also boosted to 54 Gy. Patients with stage I disease did not receive prophylactic irradiation of the inguinal nodes. Information from the baseline PET scan was used to assist in radiotherapy treatment planning.

Concurrent chemotherapy was with mitomycin C 10 mg m^–2 intravenously on day 1, and either 5-fluorouracil (5-FU) 1 g m^–2 day^–1 continuous intravenous infusion for 4 days during weeks 1 and 5 of radiotherapy or an alternative infusional 5-FU schedule of 300 mg m^–2 day^–1 for 4 days of every week of radiotherapy on a clinical trial. The median duration of chemoradiotherapy was 39 days.

Post-therapy assessment and follow-up

Patients were evaluated clinically for tumour response at regular intervals post chemoradiotherapy as per usual practice. Post-treatment FDG-PET scans were performed at a median of 69 days after the final radiotherapy fraction (range 20–255 days). As early post-therapy FDG-PET scans may be anticipated to be less specific for persistent disease due to acute radiotherapy change, and late scans delay evaluation of response and are potentially influenced by pretest selection bias for patients with symptoms or signs suggesting possible relapse, the subgroup of patients undergoing PET scans within 40–140 days post treatment (n=34, 71% cohort) were the subject of a separate analysis (Table 2). Some patients underwent two post-therapy scans because of equivocal results for residual disease in the first scan. In this group, the second scan result was utilised for analysis but only if it occurred <140 days after treatment completion, and this was the case for 7 patients (15% overall).

Long-term follow-up for determination of cancer-specific outcomes was via patient attendance at hospital clinics or through the referring physician. Final patient follow-up occurred between February 2009 and January 2010 for 29 of the 35 patients not known to have died. Median patient follow-up among these 35 patients was 5.0 years (range 1.7–9.1 years). Disease progression was confirmed on biopsy for all patients with local recurrence and imaging ±biopsy in the case of distant metastasis.

Statistical analysis

Fisher's exact tests and exact Cochran–Armitage tests for trend were used for the comparison of patients with and without post-treatment PET scans; P-values were two sided. The 95% confidence intervals were exact. Progression-free survival was measured from the date of final radiotherapy fraction until confirmed anal cancer progression or death from any cause. Overall survival and anal cancer-specific survival (CSS) were measured from the date of final radiotherapy fraction to date of death.

The PFS, OS and CSS were estimated using the Kaplan–Meier method. Univariate Cox proportional hazards regression modelling was used to determine the association of tested variables with PFS and OS. Analyses were performed using SAS (v9, SAS Institute, Cary, NC, USA).

Results

Of the 74 patients who received staging FDG-PET scans, 48 (65%) also underwent post-treatment FDG-PET assessment of tumour response. The characteristics of this group in comparison with the entire cohort are shown in Table 1. To exclude selection bias among patients undergoing both pre- and post-therapy FDG-PET scans, we compared patient demographics, T stage, N stage and stage grouping with those of the entire cohort; only T stage was shown to be significantly different (P=0.02) because of the higher representation of T2 and T4 tumours in patients undergoing both studies.

Table 1. Patient characteristics.

Baseline characteristic	All patients with baseline FDG-PET, n (%)	Patients with baseline and post-therapy FDG-PET, n (%)	P-value
No. of patients	74	48
Age (years)
Median	57	56
Range	27–88	35–87
Gender
Female	44 (59)	26 (54)	0.23
Male	30 (41)	22 (46)
T stage
T1	24 (32)	9 (19)	0.02
T2	30 (41)	24 (50)
T3	9 (12)	6 (13)
T4	11 (15)	9 (19)
N stage
N0	47 (64)	30 (63)	0.53
N1	3 (4)	2 (4)
N2	15 (20)	8 (17)
N3	9 (12)	8 (17)
M stage
M0	72	46
M1	2	2
Stage group
I	21 (28)	8 (17)	0.21
II	22 (30)	18 (38)
IIIA	7 (9)	6 (13)
IIIB	22 (30)	14 (29)
IV	2 (3)	2 (4)

Abbreviation: FDG-PET=¹⁸F-fluorodeoxyglucose positron emission tomography.

Pre- and post-chemoradiotherapy FDG-PET results

Baseline tumour staging is shown in Table 1. Pre-treatment FDG-PET results according to site were: 45 patients with FDG-avid primary lesion (94% 3 patients without an avid primary lesion due to pre-PET excisional biopsy); 15 with FDG-avid regional lymph node involvement (31%); and 2 with metastatic disease (4% 1 with para-aortic node involvement and 1 with low-volume supraclavicular and axillary lymphadenopathy. In addition, 15 patients had coincident nonanal carcinoma distant FDG-avid abnormalities; most commonly, inflammatory mediastinal lymphadenopathy consistent with granulomatous disease (6 patients, 13%) and thyroiditis (3 patients, 6%). However, for 3 of these 15 patients (6% of the cohort), the coincidental abnormality represented a synchronous malignancy: two primary lung cancers and one bladder cancer.

Metabolic responses to chemoradiotherapy are shown in Table 2. Overall, 94% of patients experienced a metabolic response to chemoradiotherapy (79% CMR, 15% PMR) in both PET scan ranges.

Table 2. Post-chemoradiotherapy metabolic responses grouped by timing of FDG-PET.

Result	All patients with post-treatment PET, n (%)	Patients with post-treatment PET in range 40–140 days, n (%)
No. of patients	48	34
Time to FDG-PET (days)
Median	69	73
Range	20–255	40–140
Primary anal tumour
Complete response	41 (85)	29 (85)
Partial response	7 (15)	5 (15)
No response	0 (0)	0 (0)
Regional lymph nodes
Complete response	48 (100)	34 (100)
Partial response	0 (0)	0 (0)
No response	0 (0)	0 (0)
Distant disease
Complete response	45 (94)	32 (94)
Partial response	0 (0)	0 (0)
No response	3 (6)	2 (6)
Overall tumour response rate
Complete response	38 (79)	27 (79)
Partial response	7 (15)	5 (15)
No response	3 (6)	2 (6)

Abbreviation: FDG-PET=¹⁸F-fluorodeoxyglucose positron emission tomography.

FDG-PET metabolic response and survival outcomes

At the time of analysis, 11 patients had experienced disease progression: 5 with local recurrence only and 6 distant relapse. The patients categorised as metabolic nonresponders (n=3) all manifested progressive distant disease, two with para-aortic lymphadenopathy only and one with liver metastases. A total of 13 patients (27%) are known to have died, 7 from anal cancer and 6 because of other causes, although nil from the coincident malignancies at baseline.

The PFS stratified for FDG-PET metabolic response is shown in Figure 1. At 2 years, estimated PFS for patients experiencing a CMR was 95% (95% CI: 88–100%), for PMR 71% (95% CI: 45–100%) and for NR 0% (95% CI: 0–71%). There was a significant difference between the three categories of PET response (P<0.0001), but not PFS according to the degree of metabolic response (CMR vs PMR; P=0.19). This analysis was repeated for the subgroup with post-therapy scans within 40–140 days with similar results; P<0.0001 for the three-arm comparison and no significant difference for CMR vs PMR (data not shown).

Figure 1.

Progression-free survival in all patients according to FDG-PET metabolic response.

Overall survival curves according to metabolic response (Figure 2) show differences in OS by metabolic response (P<0.0001 for three-arm comparison, P=0.03 for CMR vs PMR). The estimated OS rate at 5 years was 88% (95% CI: 78–100%) in patients with a CMR, 69% (95% CI: 40–100%) with PMR and 0% (95% CI: 0–71%) for no metabolic response. In the patient subgroup with PET scans within 40–140 days, OS results were again significant for the three-arm comparison (P<0.0001) but no longer reached significance for CMR vs PMR (P=0.09; data not shown). Anal CSS results stratified for metabolic response (Figure 3) were very similar to those for OS; P<0.0001 for three-arm comparison and P=0.02 for CMR vs PMR.

Figure 2.

Overall survival in all patients according to FDG-PET metabolic response.

Figure 3.

Cause-specific survival in all patients according to FDG-PET metabolic response.

Cox regression analyses

For PFS, the only significant correlation was with incomplete metabolic response to treatment (partial or no response) vs no response (HR 4.1. (95% CI: 1.5–11.5, P=0.013; Table 3). Higher T and N stage were suggestive of worse prognosis but did not reach statistical significance in this patient cohort. In prediction of OS times, FDG-PET incomplete metabolic response was again the only variable to show significant evidence of an association (HR 6.7 (95% CI: 2.1–21.6, P=0.002)).

Table 3. Cox proportional hazards regression model for factors influencing progression-free and overall survival.

Variable	Hazard ratio (95% confidence interval (CI))	χ 2	P-value
Progression-free survival
Gender (male vs female)	0.7 (0.3–1.8)	0.6	0.43
Age	1.0 (1.0–1.0)	0.0	0.93
T stage	1.5 (0.9–2.4)	2.7	0.10
N stage	1.4 (0.9–2.0)	2.6	0.11
Incomplete metabolic response	4.1 (1.5–11.5)	6.2	0.013
Overall survival
Gender (male vs female)	0.6 (0.2–1.8)	1.0	0.32
Age	1.0 (1.0–1.1)	0.4	0.52
T stage	1.6 (0.9–2.7)	3.0	0.09
N stage	1.5 (1.0–2.3)	9.0	0.08
Incomplete metabolic response	6.7 (2.1–21.6)	9.2	0.002

Incomplete metabolic response partial metabolic response (PMR) +no response (NR) vs complete metabolic response.

Discussion

The purpose of this study was to determine the utility of post-treatment FDG-PET in predicting outcomes in anal cancer managed with definitive chemoradiotherapy. To our knowledge, only one other publication has examined the value of FDG-PET in this setting, and found in a retrospective series of 53 patients (4 non-squamous histology) that metabolic response was a more significant predictor of PFS than pretreatment tumour size and nodal status (Schwarz et al, 2008). At a mean follow-up of 26 months, a significant difference in cause-specific survival rates was already evident between patients with a CMR vs PMR (2-year cause-specific survivals of 95% and 22%, respectively, P=0.0008) (Schwarz et al, 2008). Nguyen et al (2008) examined 25 patients who underwent pre- and post-chemoradiotherapy FDG-PET scans in a cohort of 50 patients retrospectively assessed for the impact of PET on their staging and management, but reported only descriptive findings; 2-year PFS was 68% in patients with CMR vs 40% in those with PMR.

A highly significant difference in PFS according to CMR, PMR or NR to chemoradiotherapy was seen in this series. If validated in other series, it could be postulated that a potential application of post-chemoradiotherapy FDG-PET is in identifying those patients with only a PMR for additional treatment, such as surgical intervention or enrolment in a clinical trial of novel therapies. Such an application of FDG-PET is the subject of current clinical trials in Hodgkin's and non-Hodgkin's lymphomas (Moskowitz et al, 2010) because of the highly prognostic nature of metabolic response during, and after, first-line treatment in these diseases (Mikhaeel et al, 2000; Kobe et al, 2008). Conversely, post-therapy FDG-PET may be of use in excluding the need for further investigation, such as biopsy, in patients with uncertain clinical findings, such as residual masses that may represent either viable tumour or radiotherapy-induced fibrosis, but a CMR. FDG-PET has proven valuable post chemoradiotherapy for SCC of the head and neck in identifying those patients who do not require a subsequent neck dissection despite residual lymphadenopathy (Porceddu et al, 2005; Nayak et al, 2007; Ong et al, 2008). In this setting, the negative predictive value of FDG-PET for residual malignancy at the primary site, within persistent cervical lymphadenopathy, and within the clinically node-negative neck is 97%, 94% and 98%, respectively (Ong et al, 2008).

Cause-specific and OS analyses found significant differences between all three categories of metabolic response and also specifically CMR vs PMR. NR patients (n=3) experienced early distant disease progression and poor survival times. The two patients in our cohort with baseline metastatic disease, although low volume and nonvisceral, were among the three nonresponders. As a result, the two-arm comparison of CMR vs PMR OS and cause-specific survival is an analysis of patients with locoregional disease only; the significant separation in the survivals of these two groups excludes the NR group as solely accounting for the highly significant P-value (P<0.0001) in the three-arm comparative results.

Of the 13 deaths in this study, 6 (46%) were not related to anal cancer, and yet did not preclude the demonstration of significantly different OS according to tumour metabolic response. Patients with only PMR or NR to chemoradiotherapy did not receive further curative-intent treatment on the basis of FDG-PET result alone because of the investigational nature of this imaging modality at the time and the presence of incurable distant disease in some patients. As such, this patient cohort provides observational data that may become limited in the future. Based on our experience in other diseases, salvage therapies are increasingly being instituted in patients with only PMR after definitive chemoradiation.

Primary tumour size and involvement of nodal basins are accepted anal cancer prognostic factors (Deniaud-Alexandre et al, 2003; Bilimoria et al, 2009; Myerson et al, 2009). These variables were recently validated prospectively in the RTOG 98–11 clinical trial cohort (Ajani et al, 2010). A recently published study in 77 patients with anal cancer found that pretreatment FDG-PET maximum standardised uptake value (SUVmax) may also confer prognostic information; higher baseline SUVmax showed borderline statistical association with disease-free survival (P=0.05); however, it was not associated with cause-specific survival (Kidd et al, 2010). In our study examining FDG-PET metabolic response, similar to Schwarz et al (2008), a greater correlation was seen with survival outcomes than for tumour T and N stages. These findings suggest that tumour metabolic response provides a valuable additional tool in prognostication. Previous studies in anal cancer have demonstrated that the clinical response within the primary anal tumour provides prognostic information (Chapet et al, 2005), and our results are consistent with this. The advantages of post-therapy FDG-PET over clinical examination, however, are the ability to simultaneously compare pre- and post-treatment assessments, ease of differentiating between abnormalities and normal tissue, and additional information provided regarding regional and distant disease status by a whole-body PET study.

The limitations of our study include its single-institution basis and retrospective nature, with resultant variability in the performance, and timing, of post-treatment imaging studies. The discrepancy in T stage between patients who underwent post-chemoradiotherapy FDG-PET scanning, compared with the whole cohort with a baseline FDG-PET study, did not create a significant stage difference between the patient groups, but may nonetheless limit the applicability of our findings to small, node-negative anal cancers. However, it could be argued that it is in patients with more advanced disease that therapeutic response assessment is most pertinent because of their higher risk of relapse. The optimal timing of post-therapy FDG-PET in anal cancer is currently unknown. In SCC of the head and neck, the negative predictive value of post-therapy metabolic response is higher than the positive predictive value because of confounding post-treatment inflammatory change (Porceddu et al, 2005; Nayak et al, 2007). In these two studies, as well as in another study (Andrade et al, 2006), later FDG-PET scans gave the greatest specificity. Because of the wide range of times to post-therapy FDG-PET scan in our study, a sensitivity analysis was conducted comparing scans within 40–140 days (71% patients) with all scans, and found identical proportions of CMR, PMR and NR in the respective groups. These findings would suggest that post-chemoradiotherapy FGD-PET findings in anal cancer are likely robust across a window period of some months; however, this issue clearly requires further investigation.

There remains scope for improvement in outcomes in both primary and salvage treatment for anal cancer. Definitive chemoradiotherapy with mitomycin and 5-FU is associated with a 5-year disease-free survival rate of 60% (Ajani et al, 2008). The current standard of care for patients with persistent or locally recurrent anal carcinoma is salvage APR, which yields 5-year OS rates, limited to patients managed with curative intent, of only 33–64% (Ellerhorn et al, 1994; Nilsson et al, 2002; Akbari et al, 2004; Mullen et al, 2006). The antiepidermal growth factor receptor (EGFR) monoclonal antibody cetuximab is in phase I clinical trial testing combined with cisplatin and 5-FU-based chemoradiotherapy (Olivatto et al, 2010). The incorporation of pre- and post-therapy FDG-PET in clinical trials of new treatment approaches in this malignancy would allow the prospective evaluation of the therapies, the significance of tumour metabolic response and appropriate timing of FDG-PET imaging.

In conclusion, this study has demonstrated the prognostic power of FDG-PET imaging in predicting survival outcomes in anal carcinoma managed with chemoradiotherapy. Given the findings of this and other studies, consideration should be given to incorporating measurement of FDG-PET metabolic response to treatment in prospective clinical trials in anal cancer.