Fig. 5.

Purification of Toolkit peptide III-30 by Gel filtration. The gel filtration of 29%-pure HPLC-purified fraction in trace (a) shows the separation of the higher mass helical component of III-30 away from the reducing agent TCEP and single chain peptide that did not fold to a helix. Polarimetry derivative traces are given for the same sample before separation (b), for re-purified HPLC III-30 (c), for Gel filtration-separated helix (d) and for single strand (e) samples after they had been given time to refold at 4 °C for 12 h. The scale bar is as for Fig. 1.