The authors of “Nutrient stress does not cause retrograde transport of cytoplasmic tRNA to the nucleus in evolutionarily diverse organisms” (Mol. Biol. Cell [2011] 22, 1091–1103) would like to make corrections to their paper. The top left-hand panel of Supplemental Figure 3 is a duplication of the top panel of Figure 3D, and the bottom left-hand panel of Supplemental Figure 3 is duplicated with the second left-hand panel of Supplemental Figure 2. Supplemental Figure 4, B–D, is the same as Figure 5, B–D, and the top and bottom panels of Figure 10B showing FISH analyses of the cellular location of tRNAGly in cells starved of amino acids are identical. The authors have replaced the duplications in Figure 10 and Supplemental Figures 3 and 4 with the correct data. The text was not changed, as the results, legends, and conclusions were not affected by the errors. The authors sincerely apologize to colleagues in the nuclear-cytoplasmic tRNA trafficking field for any inconvenience these errors may have caused.
FIGURE 10:
Loss of the function of Rna1p does not affect nuclear import of spliced tRNA during amino acid deprivation. (A) The temperature-sensitive rna1–1 mutant strain was incubated at the permissive temperature (24°C) in complete synthetic medium or synthetic medium containing glucose and lacking amino acids for 30 min. (B) rna1–1 was incubated at the nonpermissive temperature (37°C) in complete synthetic medium for 15 min to inactivate Rna1p and then switched to synthetic medium containing glucose and lacking amino acids for 30 min. The cellular location of tRNAGly, tRNATyr, and mRNA was monitored by FISH. The DNA was visualized by DAPI staining and the arrows indicate nuclear accumulation of tRNA. (C) The percentage of cells showing nuclear retention of tRNATyr in the rna1–1 strain was quantified using several different fields from three independent experiments, and the average is plotted in a bar graph.
FIGURE S3:
The amount of oligonucleotide used to detect tRNALys in rat hepatoma H4IIE starved of amino acids is not limiting, and the DIG-UTP-labeled but not the Cy3-labeled tRNALys oligonucleotide detects nuclear retention of a small amount of RNA. The cellular location of tRNALys in cells starved of amino acids in serum free DMEM media for 4 h or fed cells was monitored by FISH using 10 and 40 pmol of DIG-UTP- (left) or Cy3-labeled (right) oligonucleotide. The arrows indicate the position of nuclei.
FIGURE S4:
Glucose starvation affects nuclear-cytoplasmic tRNA trafficking in S. cerevisiae, S. paradoxus and S. bayanus but not in K. lactis. The cells were starved of glucose in synthetic medium for the times indicated and FISH was used to monitor the cellular location of tRNATyr. DAPI staining was used to observe the nucleus and the arrows indicate the location of nuclei.