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Kinetic parameters of AsV transport by zebrafish NaPi-IIb1. NaPi-IIb1–injected oocytes were incubated in ND96 buffer (pH 7.4) containing increasing concentrations of potassium arsenate (0, 0.025, 0.05, 0.1, 0.2, 0.5, and 1.0 mM) for 30 min at room temperature. Nine oocytes were used for each data point. NaPi-IIb1 transports arsenate with fairly high affinity. The Km and Vmax values for arsenate transport by zebrafish NaPi-IIb1 are 0.22 mM and 3.17 pmol/min/oocyte.
