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. Author manuscript; available in PMC: 2012 Aug 30.
Published in final edited form as: Biochemistry. 2011 Aug 8;50(34):7426–7439. doi: 10.1021/bi2006866

Table 2.

Kinetic Parameters for the Oxidation of S-(+)-Tetralol and R-(−)-Tetralol by Wild Type and Mutants of Type II Polyketide Ketoreductases (ActKR and HedKR)

graphic file with name nihms-317349-t0009.jpg graphic file with name nihms-317349-t0010.jpg
kcat (s−1) Km (mM) kcat/Km
(s−1 mM−1)		 kcat (s−1) Km (mM) kcat/Km
(s−1 mM−1)
ActKR
WT 0.24 ± 0.01 6.91 ± 1.2 0.035 ± 0.006 0.06 ± 0.004 6.47 ± 0.90 0.010 ± 0.001
P94L 0.24 ± 0.03 6.57 ± 1.8 0.036 ± 0.011 ND >>40 ND
HedKR
WT 0.168 ± 0.0109 108 ± 7.98 0.00156 ±
0.000153		 ND ND ND
N92L 0.169 ±
0.00653		 4.32 ± 0.521 0.0391 ± 0.00496 ND ND ND
N92P 0.0323 ±
0.00166		 14.1 ± 1.36 0.00229 ±
0.000249		 ND ND ND

ND: No detectable activity