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. 1989 Mar 11;17(5):1845–1863. doi: 10.1093/nar/17.5.1845

Anti-OTF-1 antibodies inhibit NFIII stimulation of in vitro adenovirus DNA replication.

J M Pruijn 1, P C van der Vliet 1, N A Dathan 1, I W Mattaj 1
PMCID: PMC317527  PMID: 2928110

Abstract

HeLa cell OTF-1 has been purified on the basis of its DNA binding activity and used to raise a polyclonal rabbit antiserum. This antiserum is shown to recognize both native and denatured OTF-1 from both human and a similar protein from Xenopus culture cells, but to react either more weakly or not at all with the lymphoid cell-specific OTF-2. Separately, NFIII has been purified on the basis of its ability to stimulate Adenovirus DNA replication in vitro. On denaturing polyacrylamide gels OTF-1 and NFIII exhibit identical mobility. Anti-OTF-1 antiserum recognizes NFIII and neutralizes its stimulatory effect on DNA replication. Moreover, OTF-1 can functionally replace NFIII. Taken together with previously published DNA binding data, this indicates that OTF-1 and NFIII are either very closely related or identical.

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Selected References

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