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. 1989 Mar 25;17(6):2181–2186. doi: 10.1093/nar/17.6.2181

Non-radioactive chemical sequencing of biotin labelled DNA.

P Richterich 1
PMCID: PMC317587  PMID: 2704617

Abstract

Methods for the nonradioactive chemical sequencing of DNA are described. A biotin marker molecule, attached chemically to an oligonucleotide primer or enzymatically in an endfilling reaction of restriction enzyme sites, is stable during the base-specific chemical modification and strand scission reactions. Following fragment separation by direct blotting electrophoresis, the membrane bound sequence pattern can be visualized by a streptavidin-bridged enzymatic color reaction. The biotin labeling is also applicable for DNA sequencing by random degradation of phosphothioates, thus showing to be a universal label for nonradioactive DNA sequencing.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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