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. 1989 Mar 25;17(6):2197–2201. doi: 10.1093/nar/17.6.2197

Polymerase chain reaction: replication errors and reliability of gene diagnosis.

M Krawczak 1, J Reiss 1, J Schmidtke 1, U Rösler 1
PMCID: PMC317589  PMID: 2565023

Abstract

The impact of replication errors on the reliability of polymerase chain reaction (PCR) data is studied theoretically. Practical applications of our results to RFLP analysis and oligonucleotide probing confirm that for practical purposes replication errors can be neglected if a large number of starting templates (e.g. 100,000) is being used. For single locus analysis in single cells, however, the probability of false diagnosis due to such errors is of the order of 1 percent.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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