(A) Images of negatively-stained, collapsed sacculi of engulfing cells.
(B) Tomographic slices through reconstructions of purified sacculi of engulfing cells. Black arrows mark the prespore PG and white arrows mark folds in the mother cell PG caused by the collapse of the sacculi on the EM grid. The inset shows a slice where the prespore PG can be seen merging with the mother cell PG at the tip.
(C) Two models for the structure and role of PG during sporulation. The key difference is the presence of PG between the IsM and OsM during engulfment, as highlighted in the red intermediate unique to the “continuous PG” model.
See also Figure S7 and Figure S8.