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. 1989 Apr 25;17(8):3199–3215. doi: 10.1093/nar/17.8.3199

Transient expression analysis of the reticuloendotheliosis virus long terminal repeat element.

A A Ridgway 1, H J Kung 1, D J Fujita 1
PMCID: PMC317723  PMID: 2542893

Abstract

A region of the Reticuloendotheliosis virus (REV) long terminal repeat (LTR) harbouring single or duplicated copies of 46-bp and 26-bp sequence elements is implicated in enhancer activity. Sequences residing upstream from the proviral 3' LTR did not contribute to activity of the intact LTR. Gene expression regulated by a combination of REV enhancer and SV40 early region promoter was 50-fold less than from the analogous construct containing the chicken syncytial virus promoter. Deletion of LTR sequences immediately downstream of the CAP site, which include a region capable of forming a stable hairpin in the mRNA, decreased expression by 70%. Expression assays and S1 nuclease mapping showed that a second transcriptional start site, identified by transcription in vitro using HeLa cell lysates and purified DNA templates, was not used in vivo in the cell lines examined.

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Selected References

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