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. 1989 May 25;17(10):3655–3661. doi: 10.1093/nar/17.10.3655

Complementation of a Dictyostelium discoideum thymidylate synthase mutation with the mouse gene provides a new selectable marker for transformation.

A C Chang 1, K L Williams 1, J G Williams 1, A Ceccarelli 1
PMCID: PMC317847  PMID: 2734099

Abstract

A cDNA encoding mouse thymidylate synthase has been inserted 3' to the Dictyostelium discoideum actin 15 promoter in an E. coli-D.discoideum shuttle vector. When this construct was introduced into a D.discoideum thymidylate synthase mutant strain HPS400, stable transformants were obtained at high frequency. These transformants grew in standard axenic medium without requiring exogenous thymidine. This construct provides a second selectable marker for use in transformation of D.discoideum.

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Selected References

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