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. 1989 May 25;17(10):3829–3843. doi: 10.1093/nar/17.10.3829

Characteristics of a multicopy gene family predominantly consisting of processed pseudogenes.

A M Van den Ouweland 1, H L Van Duijnhoven 1, K A Deichmann 1, J J Van Groningen 1, L de Leij 1, W J Van de Ven 1
PMCID: PMC317862  PMID: 2543954

Abstract

The monoclonal antibody MOC-32 detected a 40 kDa protein in Western blot analysis. Immunological screening of an expression library of human SCLC cells with MOC-32 led to the isolation of overlapping cDNA clones. One of these, cHD4, was 1.0 kbp long and of about the same size as its corresponding mRNA. Preceded by an in phase stop codon, an open reading frame of 885 bp was present in cHD4 and a translational product of only 33 kDa could be calculated. Biochemical and immunological analysis established the relationship between the 40 kDa antigen and the isolated coding sequences and resolved the apparent discrepancy between the calculated molecular weight and the observed electrophoretic mobility. Nucleotide sequence comparison of cHD4 to the EMBL database revealed that cHD4 was nearly identical to a sequence claimed to encode a laminin binding protein. Southern blot and nucleotide sequence analysis indicated the presence of multiple copies of the gene in the human genome. At least five of these appeared to represent processed pseudogenes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auffray C., Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980 Jun;107(2):303–314. doi: 10.1111/j.1432-1033.1980.tb06030.x. [DOI] [PubMed] [Google Scholar]
  2. Bepler G., Rotsch M., Jaques G., Haeder M., Heymanns J., Hartogh G., Kiefer P., Havemann K. Peptides and growth factors in small cell lung cancer: production, binding sites, and growth effects. J Cancer Res Clin Oncol. 1988;114(3):235–244. doi: 10.1007/BF00405828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hanahan D., Meselson M. Plasmid screening at high colony density. Gene. 1980 Jun;10(1):63–67. doi: 10.1016/0378-1119(80)90144-4. [DOI] [PubMed] [Google Scholar]
  4. Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
  5. Makrides S., Chitpatima S. T., Bandyopadhyay R., Brawerman G. Nucleotide sequence for a major messenger RNA for a 40 kilodalton polypeptide that is under translational control in mouse tumor cells. Nucleic Acids Res. 1988 Mar 25;16(5):2349–2349. doi: 10.1093/nar/16.5.2349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Matrisian L. M., Glaichenhaus N., Gesnel M. C., Breathnach R. Epidermal growth factor and oncogenes induce transcription of the same cellular mRNA in rat fibroblasts. EMBO J. 1985 Jun;4(6):1435–1440. doi: 10.1002/j.1460-2075.1985.tb03799.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mulders J. W., Voorter C. E., Lamers C., de Haard-Hoekman W. A., Montecucco C., van de Ven W. J., Bloemendal H., de Jong W. W. MP17, a fiber-specific intrinsic membrane protein from mammalian eye lens. Curr Eye Res. 1988 Feb;7(2):207–219. doi: 10.3109/02713688808995750. [DOI] [PubMed] [Google Scholar]
  9. Roebroek A. J., Schalken J. A., Bussemakers M. J., van Heerikhuizen H., Onnekink C., Debruyne F. M., Bloemers H. P., Van de Ven W. J. Characterization of human c-fes/fps reveals a new transcription unit (fur) in the immediately upstream region of the proto-oncogene. Mol Biol Rep. 1986;11(2):117–125. doi: 10.1007/BF00364823. [DOI] [PubMed] [Google Scholar]
  10. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schalken J. A., Ebeling S. B., Isaacs J. T., Treiger B., Bussemakers M. J., de Jong M. E., Van de Ven W. J. Down modulation of fibronectin messenger RNA in metastasizing rat prostatic cancer cells revealed by differential hybridization analysis. Cancer Res. 1988 Apr 15;48(8):2042–2046. [PubMed] [Google Scholar]
  12. Schalken J. A., van den Ouweland A. M., Bloemers H. P., van de Ven W. J. Characterization of the feline c-abl proto-oncogene. Biochim Biophys Acta. 1985 Feb 20;824(2):104–112. doi: 10.1016/0167-4781(85)90086-7. [DOI] [PubMed] [Google Scholar]
  13. Segui-Real B., Rhodes C., Yamada Y. The human genome contains a pseudogene for the Mr=32,000 laminin binding protein. Nucleic Acids Res. 1989 Feb 11;17(3):1257–1257. doi: 10.1093/nar/17.3.1257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Staden R. An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Res. 1982 May 11;10(9):2951–2961. doi: 10.1093/nar/10.9.2951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Van den Ouweland A. M., Van Groningen J. J., Schalken J. A., Van Neck H. W., Bloemers H. P., Van de Ven W. J. Genetic organization of the c-sis transcription unit. Nucleic Acids Res. 1987 Feb 11;15(3):959–970. doi: 10.1093/nar/15.3.959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Waibel R., O'Hara C. J., Stahel R. A. Characterization of an epithelial and a tumor-associated human small cell lung carcinoma glycoprotein antigen. Cancer Res. 1987 Jul 15;47(14):3766–3770. [PubMed] [Google Scholar]
  18. Wewer U. M., Liotta L. A., Jaye M., Ricca G. A., Drohan W. N., Claysmith A. P., Rao C. N., Wirth P., Coligan J. E., Albrechtsen R. Altered levels of laminin receptor mRNA in various human carcinoma cells that have different abilities to bind laminin. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7137–7141. doi: 10.1073/pnas.83.19.7137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wewer U. M., Taraboletti G., Sobel M. E., Albrechtsen R., Liotta L. A. Role of laminin receptor in tumor cell migration. Cancer Res. 1987 Nov 1;47(21):5691–5698. [PubMed] [Google Scholar]
  20. Yamamoto T., Davis C. G., Brown M. S., Schneider W. J., Casey M. L., Goldstein J. L., Russell D. W. The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell. 1984 Nov;39(1):27–38. doi: 10.1016/0092-8674(84)90188-0. [DOI] [PubMed] [Google Scholar]
  21. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]
  22. Young R. A., Davis R. W. Efficient isolation of genes by using antibody probes. Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194–1198. doi: 10.1073/pnas.80.5.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yow H. K., Wong J. M., Chen H. S., Lee C. G., Davis S., Steele G. D., Jr, Chen L. B. Increased mRNA expression of a laminin-binding protein in human colon carcinoma: complete sequence of a full-length cDNA encoding the protein. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6394–6398. doi: 10.1073/pnas.85.17.6394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. de Leij L., Poppema S., Nulend J. K., ter Haar A., Schwander E., Ebbens F., Postmus P. E., The T. H. Neuroendocrine differentiation antigen on human lung carcinoma and Kulchitski cells. Cancer Res. 1985 May;45(5):2192–2200. [PubMed] [Google Scholar]
  25. de Leij L., Postmus P. E., Buys C. H., Elema J. D., Ramaekers F., Poppema S., Brouwer M., van der Veen A. Y., Mesander G., The T. H. Characterization of three new variant type cell lines derived from small cell carcinoma of the lung. Cancer Res. 1985 Dec;45(12 Pt 1):6024–6033. [PubMed] [Google Scholar]

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