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. Author manuscript; available in PMC: 2012 Jul 19.
Published in final edited form as: Oncogene. 2011 Jun 20;31(3):322–332. doi: 10.1038/onc.2011.236

Figure 4. Additional deletion of either one or two copies of Pten accelerates tumor formation and leads to lymph node metastases.

Figure 4

(A) H&E stains. Examples of rare, focal HGPIN in old Pten-het;p53-ko mouse prostate (age=50 weeks) are shown in panels `a–d'. (B) H&E stains of sections from c-MYC+;Ptenhet;p53-ko mice show aggressive cancers (panels `a' and `b') with lymph node metastases (panels `c–e'). `c,d,e' show H&E, staining for androgen receptor (AR) and cytokeratin 8 (CK8) in lymph node (insets are higher magnification images). (C) Immunofluorescent detection of focal (a) or diffuse (b) loss of Pten expression (arrows) in mice with the indicated genotypes. (D) H&E stained sections show HGPIN/well-differentiated cancer and moderately-differentiated cancer in Pten-ko;p53-het (a) and c-MYC+;Pten-ko;p53-het (b) mouse prostates, respectively. Scale bars: 100μm.