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. 1989 Jun 26;17(12):4769–4782. doi: 10.1093/nar/17.12.4769

Regiospecific inhibition of DNA duplication by antisense phosphate-methylated oligodeoxynucleotides.

H M Moody 1, M H van Genderen 1, L H Koole 1, H J Kocken 1, E M Meijer 1, H M Buck 1
PMCID: PMC318031  PMID: 2748337

Abstract

A new synthesis route for long phosphate-methylated oligodeoxynucleotides is described, which were used as antisense inhibitors of the DNA replication. Phosphate-methylated oligomers hybridize more strongly with natural DNA than their natural analogues, due to the absence of electrostatic interstrand repulsions. Compared with phosphate-ethylated and methyl phosphonate systems, phosphate-methylated systems are preferable as antisense DNA, which was concluded from the high Tm values and sharp melting transitions of duplexes of phosphate-methylated and natural DNA. By using the Sanger dideoxy technique, it was shown that a complementary phosphate-methylated 18-mer can effectively and site-specifically block the DNA replication process at room temperature.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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