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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Bone. 2011 Apr 29;50(2):568–575. doi: 10.1016/j.bone.2011.04.017

Figure 6. Daily injections of osteocalcin prevent liver steatosis in mice fed a high-fat diet.

Figure 6

Analyses were performed in mice fed a normal diet (ND) or a high-fat diet (HFD) for 8 weeks and then injected daily with 30 ng/g/day of osteocalcin (OCN) or with vehicle for 14 weeks. (A) Appearance of the dissected liver after 14 weeks of treatment. (B) Oil Red O staining of liver sections (magnification 100X). (C) Histomorphometric quantification of the Oil Red O positive area in the liver. Quantification was performed on 3 different fields per animals and a total of 4 animals per group were analyzed. (D) Relative expression of Tnfa in liver normalized to β-actin (real time PCR). Four animals per group were analyzed. #: p<0.05, ###: p<0.001 when comparing HFD + Vehicle and HFD + OCN groups. *: p<0.05, ***: p<0.001 when comparing to ND + Vehicle.