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. 1989 Jul 25;17(14):5501–5507. doi: 10.1093/nar/17.14.5501

Translational reinitiation in the presence and absence of a Shine and Dalgarno sequence.

R A Spanjaard 1, J van Duin 1
PMCID: PMC318173  PMID: 2668889

Abstract

The process of translational reinitiation in Escherichia coli was studied in a two cistron system where expression of the downstream reporter gene was dependent on translation of an upstream reading frame. The dependence was almost absolute. Upstream translation increased expression of the downstream gene by two to three orders of magnitude. This large difference allowed us to quantitate restarts in a meaningful manner. In the absence of a Shine and Dalgarno (SD) region reinitiation occurred but its efficiency was about 10% of that found in the SD carrying counterpart. We discuss three ways by which translational coupling between neighboring cistrons can be enforced.

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Selected References

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