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. 1989 Jul 25;17(14):5597–5610. doi: 10.1093/nar/17.14.5597

Presence of multiple species of polypeptides immunologically related to transcription factor TFIIIA in adult Xenopus tissues.

W Yasui 1, M Ryoji 1
PMCID: PMC318182  PMID: 2762150

Abstract

Transcription of 5S RNA gene in Xenopus oocytes requires a 38 kDa transcription factor TFIIIA, which interacts with the 50 bp internal control region of the gene. We looked for TFIIIA-like polypeptides in the extracts of adult Xenopus tissues on the basis of their antigenic cross-reactivity to anti-TFIIIA antibody. Several species of polypeptides ranging from 30 to 50 kDa were found in kidney, stomach, liver and testis. Although these polypeptides reacted specifically to anti-TFIIIA antibody, proteolytic peptide mapping of three representative ones did not reveal any mutual similarities. They also seemed to be distinct from TFIIIA. Possible functions of these proteins are discussed.

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