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. 1989 Jul 25;17(14):5651–5664. doi: 10.1093/nar/17.14.5651

Transcription of the chicken myb proto-oncogene starts within a CpG island.

M Dvorák 1, P Urbánek 1, P Bartůnĕk 1, V Paces 1, J Vlach 1, V Pecenka 1, L Arnold 1, M Trávnicek 1, J Ríman 1
PMCID: PMC318186  PMID: 2548166

Abstract

The nucleotide sequence of an 8.2-kb DNA fragment from the 5' proximal part of the chicken myb proto-oncogene spanning 1761 nucleotides upstream and 6436 nucleotides downstream from a presumed c-myb initiation codon was determined. A 3.3-kb G + C-rich region found in this sequence had also other features characterizing CpG islands, i.e. no CpG underrepresentation and lack of CpG methylation. In haematopoietic tissues c-myb mRNA synthesis starts in two major regions of the CpG island, namely 98 to 108 and 143 to 145 nucleotides upstream from the c-myb initiation codon. These two regions are in or close to the 124-bp evolutionarily conserved element located in the middle part of the CpG island. No alternative splicing of the 5' end of c-myb mRNA suggested earlier (1,2) was observed. The c-myb promoter contains neither TATA nor CAAT box-like structures at the usual positions. Instead, numerous potential Sp1 factor binding sites were found both upstream and downstream from the transcription initiation sites. Moreover, consensus v-myb protein DNA-binding sites were revealed in the promoter region and in sequences downstream from it.

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Selected References

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