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. 1989 Jul 25;17(14):5679–5685. doi: 10.1093/nar/17.14.5679

Mutagenesis analysis of a self-cleaving RNA.

C C Sheldon 1, R H Symons 1
PMCID: PMC318188  PMID: 2762152

Abstract

The hammerhead structural model proposed for sequences that mediate self-cleavage of certain RNAs contains base-paired three stems and 13 conserved bases. Insertion, deletion and base substitution mutations were carried out on a 58 base RNA containing the sequence of the single-hammerhead structure of the plus RNA of the virusoid of lucerne transient streak virus, and the effects on self-cleavage assessed. Results showed that there is flexibility in the sequence requirements for self-cleavage in vitro, but alterations of the conserved sequence or predicted secondary structure generally reduced the efficiency of self-cleavage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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