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. 1989 Aug 11;17(15):5923–5931. doi: 10.1093/nar/17.15.5923

Altered mRNA cap recognition activity of initiation factor 4E in the yeast cell cycle division mutant cdc33.

M Altmann 1, H Trachsel 1
PMCID: PMC318250  PMID: 2671936

Abstract

The mutation in the S. cerevisiae cell cycle division mutant cdc33 consists of a single G to A transition in the open reading frame encoding translation initiation factor 4E (eIF-4E). This leads to the substitution of glycine 113 by aspartic acid close to tryptophane 115 in the protein. This mutation reduces cap binding activity of eIF-4E as measured by binding of eIF-4E to m7GDP agarose columns and slows down overall protein synthesis at the non-permissive temperature. Comparison of the cdc33 mutation with other mutations affecting eIF-4E function supports the view that tryptophane residues and their flanking regions are involved in cap binding activity of eIF-4E.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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