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. 1989 Aug 11;17(15):6103–6108. doi: 10.1093/nar/17.15.6103

Chromosomal localization of nucleic acid-binding proteins by affinity mapping: assignment of the IRE-binding protein gene to human chromosome 9.

M W Hentze 1, H N Seuanez 1, S J O'Brien 1, J B Harford 1, R D Klausner 1
PMCID: PMC318264  PMID: 2771641

Abstract

Three human mRNAs are regulated post-transcriptionally by iron via iron-responsive elements (IREs) contained in each mRNA. A cytoplasmic protein (IRE-BP) binds to these cis-acting elements and mediates the translational regulation of ferritin H- and L-chain mRNA and the iron-dependent stability of transferrin receptor (TfR) mRNA. We have taken advantage of the different mobilities of the human and rodent IRE/IRE-BP complexes on non-denaturing polyacrylamide gels to determine the chromosomal localization of the gene encoding the IRE-BP. Utilizing a panel of 34 different human/rodent hybrid cell lines we have assigned the IRE-BP gene to human chromosome 9. This new technique based on nucleic acid/protein interaction may allow determination of the chromosomal localization of other RNA- or DNA-binding proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aziz N., Munro H. N. Iron regulates ferritin mRNA translation through a segment of its 5' untranslated region. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8478–8482. doi: 10.1073/pnas.84.23.8478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Casey J. L., Hentze M. W., Koeller D. M., Caughman S. W., Rouault T. A., Klausner R. D., Harford J. B. Iron-responsive elements: regulatory RNA sequences that control mRNA levels and translation. Science. 1988 May 13;240(4854):924–928. doi: 10.1126/science.2452485. [DOI] [PubMed] [Google Scholar]
  3. Cook P. J., Robson E. B., Buckton K. E., Slaughter C. A., Gray J. E., Blank C. E., James F. E., Ridler M. A., Insley J., Hultén M. Segregation of ABO, AK1 and ACONs in families with abnormalities of chromosome 9. Ann Hum Genet. 1978 Jan;41(3):365–377. doi: 10.1111/j.1469-1809.1978.tb01904.x. [DOI] [PubMed] [Google Scholar]
  4. Hentze M. W., Caughman S. W., Casey J. L., Koeller D. M., Rouault T. A., Harford J. B., Klausner R. D. A model for the structure and functions of iron-responsive elements. Gene. 1988 Dec 10;72(1-2):201–208. doi: 10.1016/0378-1119(88)90145-x. [DOI] [PubMed] [Google Scholar]
  5. Hentze M. W., Caughman S. W., Rouault T. A., Barriocanal J. G., Dancis A., Harford J. B., Klausner R. D. Identification of the iron-responsive element for the translational regulation of human ferritin mRNA. Science. 1987 Dec 11;238(4833):1570–1573. doi: 10.1126/science.3685996. [DOI] [PubMed] [Google Scholar]
  6. Hentze M. W., Keim S., Papadopoulos P., O'Brien S., Modi W., Drysdale J., Leonard W. J., Harford J. B., Klausner R. D. Cloning, characterization, expression, and chromosomal localization of a human ferritin heavy-chain gene. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7226–7230. doi: 10.1073/pnas.83.19.7226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hentze M. W., Rouault T. A., Caughman S. W., Dancis A., Harford J. B., Klausner R. D. A cis-acting element is necessary and sufficient for translational regulation of human ferritin expression in response to iron. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6730–6734. doi: 10.1073/pnas.84.19.6730. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kravitz K., Skolnick M., Cannings C., Carmelli D., Baty B., Amos B., Johnson A., Mendell N., Edwards C., Cartwright G. Genetic linkage between hereditary hemochromatosis and HLA. Am J Hum Genet. 1979 Sep;31(5):601–619. [PMC free article] [PubMed] [Google Scholar]
  9. Leibold E. A., Munro H. N. Cytoplasmic protein binds in vitro to a highly conserved sequence in the 5' untranslated region of ferritin heavy- and light-subunit mRNAs. Proc Natl Acad Sci U S A. 1988 Apr;85(7):2171–2175. doi: 10.1073/pnas.85.7.2171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. O'Brien S. J., Nash W. G. Genetic mapping in mammals: chromosome map of domestic cat. Science. 1982 Apr 16;216(4543):257–265. doi: 10.1126/science.7063884. [DOI] [PubMed] [Google Scholar]
  11. O'Brien S. J., Nash W. G., Goodwin J. L., Lowy D. R., Chang E. H. Dispersion of the ras family of transforming genes to four different chromosomes in man. Nature. 1983 Apr 28;302(5911):839–842. doi: 10.1038/302839a0. [DOI] [PubMed] [Google Scholar]
  12. Rouault T. A., Hentze M. W., Caughman S. W., Harford J. B., Klausner R. D. Binding of a cytosolic protein to the iron-responsive element of human ferritin messenger RNA. Science. 1988 Sep 2;241(4870):1207–1210. doi: 10.1126/science.3413484. [DOI] [PubMed] [Google Scholar]
  13. Worwood M., Brook J. D., Cragg S. J., Hellkuhl B., Jones B. M., Perera P., Roberts S. H., Shaw D. J. Assignment of human ferritin genes to chromosomes 11 and 19q13.3----19qter. Hum Genet. 1985;69(4):371–374. doi: 10.1007/BF00291657. [DOI] [PubMed] [Google Scholar]

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